ArticleApplied and environmental microbiology2015
A novel CO-responsive transcriptional regulator and enhanced H2 production by an engineered Thermococcus onnurineus NA1 strain.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Carbon monoxide-driven proton respiration enables facultative anaerobes to survive electron acceptor limitation.The ISME journal · 2026Article
- Ortholog Analysis and Transformation of Glycoside Hydrolase Genes in Hyperthermophilic ArchaealInternational journal of molecular sciences · 2025Article
- Characterization of a MHYT domain-coupled transcriptional regulator that responds to carbon monoxide.Nucleic acids research · 2024Article
- Article
- Extremophiles in a changing world.Extremophiles : life under extreme conditions · 2024Review
- NADPApplied and environmental microbiology · 2023Article
- Carbon Monoxide-Sensing Transcription Factors: Regulators of Microbial Carbon Monoxide Oxidation Pathway Gene Expression.Journal of bacteriology · 2023Review
- Bioconversion of CO to formate by artificially designed carbon monoxide:formate oxidoreductase in hyperthermophilic archaea.Communications biology · 2022Article
- A Novel NADP-Dependent Formate Dehydrogenase From the Hyperthermophilic ArchaeonFrontiers in microbiology · 2022Article
- Biohydrogen production beyond the Thauer limit by precision design of artificial microbial consortia.Communications biology · 2020Article
- Transcriptome analysis of a thermophilic and hydrogenogenic carboxydotroph Carboxydothermus pertinax.Extremophiles : life under extreme conditions · 2019Article
- Biohydrogen production of obligate anaerobic archaeonBiotechnology for biofuels · 2019Article
- Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate.Scientific reports · 2017Article
- Article
- Genetic dissection of independent and cooperative transcriptional activation by the LysR-type activator ThnR at close divergent promoters.Scientific reports · 2016Article
- Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis.Scientific reports · 2016Article
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome analysis revealed the existence of a putative transcriptional regulatory system governing CO metabolism in Thermococcus onnurineus NA1, a carboxydotrophic hydrogenogenic archaeon. The regulatory system is composed of CorQ with a 4-vinyl reductase domain and CorR with a DNA-binding domain of the LysR-type transcriptional regulator family in close proximity to the CO dehydrogenase (CODH) gene cluster. Homologous genes of the CorQR pair were also found in the genomes of Thermococcus species and "Candidatus Korarchaeum cryptofilum" OPF8. In-frame deletion of either corQ or corR caused a severe impairment in CO-dependent growth and H2 production. When corQ and corR deletion mutants were complemented by introducing the corQR genes under the control of a strong promoter, the mRNA and protein levels of the CODH gene were significantly increased in a ΔCorR strain complemented with integrated corQR (ΔCorR/corQR(↑)) compared with those in the wild-type strain. In addition, the ΔCorR/corQR(↑) strain exhibited a much higher H2 production rate (5.8-fold) than the wild-type strain in a bioreactor culture. The H2 production rate (191.9 mmol liter(-1) h(-1)) and the specific H2 production rate (249.6 mmol g(-1) h(-1)) of this strain were extremely high compared with those of CO-dependent H2-producing prokaryotes reported so far. These results suggest that the corQR genes encode a positive regulatory protein pair for the expression of a CODH gene cluster. The study also illustrates that manipulation of the transcriptional regulatory system can improve biological H2 production.
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