ArticleApplied and environmental microbiology2015
Exploring the diversity of Listeria monocytogenes biofilm architecture by high-throughput confocal laser scanning microscopy and the predominance of the honeycomb-like morphotype.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 138 citations in OpenAlex.
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- PDADMAC/Alginate-Coated Gold Nanorod For Eradication of Staphylococcus Aureus Biofilms.International journal of nanomedicine · 2024Article
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- The ribonuclease PNPase is a key regulator of biofilm formation in Listeria monocytogenes and affects invasion of host cells.NPJ biofilms and microbiomes · 2023Article
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- Effect of storage temperature and produce type on the survival or growth ofFrontiers in microbiology · 2023Article
- Exploring the Diversity of Biofilm Formation by the Food SpoilerMicroorganisms · 2022Article
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- Andrographolide Inhibits Biofilm and Virulence inMolecules (Basel, Switzerland) · 2022Article
- Architectural Features and Resistance to Food-Grade Disinfectants inFrontiers in microbiology · 2022Article
- Colonisation dynamics of Listeria monocytogenes strains isolated from food production environments.Scientific reports · 2021Article
- Biofilm Formation byApplied and environmental microbiology · 2021Article
- Morphogenesis and oxygen dynamics in phototrophic biofilms growing across a gradient of hydraulic conditions.iScience · 2021Article
- Biofilm-isolated Listeria monocytogenes exhibits reduced systemic dissemination at the early (12-24 h) stage of infection in a mouse model.NPJ biofilms and microbiomes · 2021Article
- Review
- Characterization of Binary Biofilms ofFrontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes is involved in food-borne illness with a high mortality rate. The persistence of the pathogen along the food chain can be associated with its ability to form biofilms on inert surfaces. While most of the phenotypes associated with biofilms are related to their spatial organization, most published data comparing biofilm formation by L. monocytogenes isolates are based on the quantitative crystal violet assay, which does not give access to structural information. Using a high-throughput confocal-imaging approach, the aim of this work was to decipher the structural diversity of biofilms formed by 96 L. monocytogenes strains isolated from various environments. Prior to large-scale analysis, an experimental design was created to improve L. monocytogenes biofilm formation in microscopic-grade microplates, with special emphasis on the growth medium composition. Microscopic analysis of biofilms formed under the selected conditions by the 96 isolates revealed only weak correlation between the genetic lineages of the isolates and the structural properties of the biofilms. However, a gradient in their geometric descriptors (biovolume, mean thickness, and roughness), ranging from flat multilayers to complex honeycomb-like structures, was shown. The dominant honeycomb-like morphotype was characterized by hollow voids hosting free-swimming cells and localized pockets containing mixtures of dead cells and extracellular DNA (eDNA).
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Registered trials
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