ArticleApplied microbiology and biotechnology2015
Cloning, expression, and purification of insect (Sitophilus oryzae) alpha-amylase, able to digest granular starch, in Yarrowia lipolytica host.
Article in Applied microbiology and biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- Structure and Function of Honeybee α-Amylase of Glycoside Hydrolase Family 13 Subfamily 15.Molecules (Basel, Switzerland) · 2026Article
- Exploring Plant α-Amylase Inhibitors: Mechanisms and Potential Application for Insect Pest Control.Biotechnology journal · 2025Review
- New Yarrowia lipolytica chassis strains for industrial enzyme production.Microbial cell factories · 2025Article
- 'Small volume-big problem': culturing Yarrowia lipolytica in high-throughput micro-formats.Microbial cell factories · 2024Review
- Review
- Insect α-Amylases and Their Application in Pest Management.Molecules (Basel, Switzerland) · 2023Review
- Hyperosmolarity adversely impacts recombinant protein synthesis by Yarrowia lipolytica-molecular background revealed by quantitative proteomics.Applied microbiology and biotechnology · 2022Article
- Thermal treatment improves a process of crude glycerol valorization for the production of a heterologous enzyme byBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Optimization of Yarrowia lipolytica-based consolidated biocatalyst through synthetic biology approach: transcription units and signal peptides shuffling.Applied microbiology and biotechnology · 2020Article
- Production of Raw Starch-Digesting Amylolytic Preparation inMicroorganisms · 2020Article
- Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in process conditions.Microbial cell factories · 2019Article
- Rapid micro-assays for amylolytic activities determination: customization and validation of the tests.Applied microbiology and biotechnology · 2019Article
- Article
- Bioreactor-Scale Strategies for the Production of Recombinant Protein in the YeastMicroorganisms · 2019Review
- Filamentous fungi-like secretory pathway strayed in a yeast system: peculiarities of Yarrowia lipolytica secretory pathway underlying its extraordinary performance.Applied microbiology and biotechnology · 2019Review
- Robust signal peptides for protein secretion in Yarrowia lipolytica: identification and characterization of novel secretory tags.Applied microbiology and biotechnology · 2018Article
- The Amylases of Insects.International journal of insect science · 2018Review
- Evaluation of a recombinant insect-derived amylase performance in simultaneous saccharification and fermentation process with industrial yeasts.Applied microbiology and biotechnology · 2016Article
- Engineering Yarrowia lipolytica to produce biodiesel from raw starch.Biotechnology for biofuels · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Raw-starch-digesting enzymes (RSDE) are of major importance for industrial applications, as their usage greatly simplifies the starch processing pipeline. To date, only microbial RSDE have gained considerable attention, since only microbial production of enzymes meets industrial demands. In this study, α-amylase from rice weevil (Sitophilus oryzae), the major rice pest, was cloned and expressed in Yarrowia lipolytica Po1g strain. The enzyme was secreted into the culture medium, and the peak activity (81 AU/L) was reached after only 29 h of culturing in 5-L bioreactors. Through simple purification procedure of ammonium sulfate precipitation and affinity chromatography, it was possible to purify the enzyme to apparent homogeneity (25-fold purification factor, at 5 % yield). The optimal conditions for the α-amylase activity were pH 5.0 and a temperature of 40 °C. The α-amylase studied here did not show any obligate requirement for Ca(2+) ions. The recombinant α-amylase appeared to efficiently digest granular starch from pea, amaranth, waxy corn, and waxy rice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.