Evidence map›Paper›PMID 25547795›Full record

ArticleInfection and immunity2015

Identification of host proteins involved in rickettsial invasion of tick cells.

Natthida Petchampai, Piyanate Sunyakumthorn, Kaikhushroo H Banajee, Victoria I Verhoeve, Michael T Kearney, Kevin R Macaluso

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Mediterranean Spotted Fever: Current Knowledge and Recent Advances.Tropical medicine and infectious disease · 2021
    Review
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Natthida PetchampaiVector-borne Disease Laboratories, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA npetchampai@vetmed.lsu.edu.
Piyanate SunyakumthornVector-borne Disease Laboratories, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA.
Kaikhushroo H BanajeeVector-borne Disease Laboratories, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA.
Victoria I VerhoeveVector-borne Disease Laboratories, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA.
Michael T KearneyVector-borne Disease Laboratories, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA.
Kevin R MacalusoVector-borne Disease Laboratories, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA.
Louisiana State University · US

Funding

Molecular basis for spotted fever group Rickettsia vector competence in ticksR01AI077784 · NIAID · UNIVERSITY OF SOUTH ALABAMA · PI MACALUSO, KEVIN R. · 2009 to 2019
$3.5M
Research Training in Experimental Medicine and PathologyT32OD011124 · OD · TULANE UNIVERSITY OF LOUISIANA · PI BLAIR, ROBERT, RAPPAPORT, JAY · 2012 to 2019
$2.7M
Research Training in Experimental Medicine and PathologyT32RR021309 · NCRR · TULANE UNIVERSITY OF LOUISIANA · PI LACKNER, ANDREW A · 2005 to 2011
$2.3M
NCRR NIH HHS T32 RR021309NIAID NIH HHS AI077784NIAID NIH HHS R01 AI077784NIH HHS T32 OD011124
6 · The paper itself

Abstract

Tick-borne spotted fever group (SFG) Rickettsia species are obligate intracellular bacteria capable of infecting both vertebrate and invertebrate host cells, an essential process for subsequent bacterial survival in distinct hosts. The host cell signaling molecules involved in the uptake of Rickettsia into mammalian and Drosophila cells have been identified; however, invasion into tick cells is understudied. Considering the movement of SFG Rickettsia between vertebrate and invertebrate hosts, the hypothesis is that conserved mechanisms are utilized for host cell invasion. The current study employed biochemical inhibition assays to determine the tick proteins involved in Rickettsia montanensis infection of tick-derived cells from a natural host, Dermacentor variabilis. The results revealed several tick proteins important for rickettsial invasion, including actin filaments, actin-related protein 2/3 complex, phosphatidylinositol-3'-kinase, protein tyrosine kinases (PTKs), Src family PTK, focal adhesion kinase, Rho GTPase Rac1, and neural Wiskott-Aldrich syndrome protein. Delineating the molecular mechanisms of rickettsial infection is critical to a thorough understanding of rickettsial transmission in tick populations and the ecology of tick-borne rickettsial diseases.

Indexed as

Host-Pathogen InteractionsActin CytoskeletonActin-Related Protein 2-3 ComplexAnimalsArthropod ProteinsDermacentorEnzyme AssaysFocal Adhesion Protein-Tyrosine KinasesGene Expression RegulationPhosphatidylinositol 3-KinasesProtein-Tyrosine Kinasesrac1 GTP-Binding ProteinRickettsiaSignal Transductionsrc-Family KinasesWiskott-Aldrich Syndrome ProteinActin-Related Protein 2-3 ComplexArthropod ProteinsFocal Adhesion Protein-Tyrosine KinasesPhosphatidylinositol 3-KinasesProtein-Tyrosine Kinasesrac1 GTP-Binding Proteinsrc-Family KinasesWiskott-Aldrich Syndrome Protein

Identifiers

PMID25547795
PMCPMC4333443
OpenAlexW2034898510

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.