ArticleVirology journal2014
Identification of putative interactions between swine and human influenza A virus nucleoprotein and human host proteins.
Article in Virology journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 34 citations in OpenAlex.
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- Viral polymerase-host interaction analysis reveals that the association between avian NUP93 and PB1 promotes H5N6 avian influenza virus replication.Microbiology spectrum · 2025Article
- Transcriptome analysis of salivary glands of rabies-virus-infected mice.Frontiers in microbiology · 2024Article
- Identification of Human Host Substrates of the SARS-CoV-2 MACS infectious diseases · 2023Article
- Characterization of host substrates of SARS-CoV-2 main protease.Frontiers in microbiology · 2023Review
- Genome-wide association analyses of common infections in a large practice-based biobank.BMC genomics · 2022Article
- Characterising proteolysis during SARS-CoV-2 infection identifies viral cleavage sites and cellular targets with therapeutic potential.Nature communications · 2021Article
- Prediction of Non-canonical Routes for SARS-CoV-2 Infection in Human Placenta Cells.Frontiers in molecular biosciences · 2021Article
- An Integrated Analysis Reveals Geniposide Extracted FromFrontiers in pharmacology · 2021Article
- Alternative Experimental Models for Studying Influenza Proteins, Host-Virus Interactions and Anti-Influenza Drugs.Pharmaceuticals (Basel, Switzerland) · 2019Review
- Sliding window haplotype approaches overcome single SNP analysis limitations in identifying genes for meat tenderness in Nelore cattle.BMC genetics · 2019Article
- Recent trends in click chemistry as a promising technology for virus-related research.Virus research · 2018Review
- Amino Acid Substitution K470R in the Nucleoprotein Increases the Virulence of H5N1 Influenza A Virus in Mammals.Frontiers in microbiology · 2017Article
- Histone Deacetylase 1 Plays an Acetylation-Independent Role in Influenza A Virus Replication.Frontiers in immunology · 2017Article
- Influenza A Virus-induced expression of ISG20 inhibits viral replication by interacting with nucleoprotein.Virus genes · 2016Article
- Article
- Proteomics Analysis of Cellular Proteins Co-Immunoprecipitated with Nucleoprotein of Influenza A Virus (H7N9).International journal of molecular sciences · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInfluenza A viruses (IAVs) are important pathogens that affect the health of humans and many additional animal species. IAVs are enveloped, negative single-stranded RNA viruses whose genome encodes at least ten proteins. The IAV nucleoprotein (NP) is a structural protein that associates with the viral RNA and is essential for virus replication. Understanding how IAVs interact with host proteins is essential for elucidating all of the required processes for viral replication, restrictions in species host range, and potential targets for antiviral therapies.
methodsIn this study, the NP from a swine IAV was cloned into a yeast two-hybrid "bait" vector for expression of a yeast Gal4 binding domain (BD)-NP fusion protein. This "bait" was used to screen a Y2H human HeLa cell "prey" library which consisted of human proteins fused to the Gal4 protein's activation domain (AD). The interaction of "bait" and "prey" proteins resulted in activation of reporter genes.
resultsSeventeen positive bait-prey interactions were isolated in yeast. All of the "prey" isolated also interact in yeast with a NP "bait" cloned from a human IAV strain. Isolation and sequence analysis of the cDNAs encoding the human prey proteins revealed ten different human proteins. These host proteins are involved in various host cell processes and structures, including purine biosynthesis (PAICS), metabolism (ACOT13), proteasome (PA28B), DNA-binding (MSANTD3), cytoskeleton (CKAP5), potassium channel formation (KCTD9), zinc transporter function (SLC30A9), Na+/K+ ATPase function (ATP1B1), and RNA splicing (TRA2B).
conclusionsTen human proteins were identified as interacting with IAV NP in a Y2H screen. Some of these human proteins were reported in previous screens aimed at elucidating host proteins relevant to specific viral life cycle processes such as replication. This study extends previous findings by suggesting a mechanism by which these host proteins associate with the IAV, i.e., physical interaction with NP. Furthermore, this study revealed novel host protein-NP interactions in yeast.
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