ArticleCytoskeleton (Hoboken, N.J.)2014
Endothelial monolayers and transendothelial migration depend on mechanical properties of the substrate.
Article in Cytoskeleton (Hoboken, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- The Alzheimer's Disease-associated gene CD2AP regulates permeability of endothelial cells and transendothelial migration of immune cells.bioRxiv : the preprint server for biology · 2026Article
- The Kallikrein-Kinin System: Proteolytic Orchestrators of Tissue Barrier Disruption in Inflammation and Cancer.International journal of molecular sciences · 2026Review
- Junctions at the crossroads: the impact of mechanical cues on endothelial cell-cell junction conformations and vascular permeability.American journal of physiology. Cell physiology · 2024Review
- Conditions that promote transcellular neutrophil migration in vivo.Scientific reports · 2024Article
- Tumour associated endothelial cells: origin, characteristics and role in metastasis and anti-angiogenic resistance.Frontiers in physiology · 2023Review
- Integration of immune cells in organs-on-chips: a tutorial.Frontiers in bioengineering and biotechnology · 2023Review
- Endothelial inflammation and neutrophil transmigration are modulated by extracellular matrix composition in an inflammation-on-a-chip model.Scientific reports · 2022Article
- Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction.Frontiers in cell and developmental biology · 2022Review
- Review of Design Considerations for Brain-on-a-Chip Models.Micromachines · 2021Review
- Uveal melanoma cells use ameboid and mesenchymal mechanisms of cell motility crossing the endothelium.Molecular biology of the cell · 2021Article
- Quantitatively relating brain endothelial cell-cell junction phenotype to global and local barrier properties under varied culture conditions via the Junction Analyzer Program.Fluids and barriers of the CNS · 2020Article
- Subendothelial stiffness alters endothelial cell traction force generation while exerting a minimal effect on the transcriptome.Scientific reports · 2019Article
- The secreted kinase ROP17 promotes Toxoplasma gondii dissemination by hijacking monocyte tissue migration.Nature microbiology · 2019Article
- Tumor Cell Mechanosensing During Incorporation into the Brain Microvascular Endothelium.Cellular and molecular bioengineering · 2019Article
- The Role of PPARβ/δ in Melanoma Metastasis.International journal of molecular sciences · 2018Article
- A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells.Journal of visualized experiments : JoVE · 2018Article
- Matrix stiffness modulates infection of endothelial cells by Listeria monocytogenes via expression of cell surface vimentin.Molecular biology of the cell · 2018Article
- A microfluidic platform for modeling metastatic cancer cell matrix invasion.Biofabrication · 2017Article
- Surface engineering for lymphocyte programming.Advanced drug delivery reviews · 2017Review
- Trojan Horse Transit Contributes to Blood-Brain Barrier Crossing of a Eukaryotic Pathogen.mBio · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Endothelial cells (ECs) line the microvasculature and constitute a barrier between the vessel lumen and surrounding tissues. ECs inform circulating immune cells of the health and integrity of surrounding tissues, recruiting them in response to pathogens and tissue damage. ECs play an active role in the transmigration of immune cells across the vessel wall. We have discovered important differences in the properties of ECs on soft hydrogel substrates of varying stiffness, in comparison to glass. Primary ECs from several human sources were tested; all formed monolayers normally on soft substrates. EC monolayers formed more mature cell-cell junctions on soft substrates, relative to glass, based on increased recruitment of vinculin and F-actin. EC monolayers supported transendothelial migration (TEM) on soft substrates. Immune cells, including peripheral blood lymphocytes (PBLs) and natural killer cells, showed decreasing numbers of paracellular (between-cell) transmigration events with decreasing substrate stiffness, while the number of transcellular (through-cell) events increased for PBLs. Melanoma cancer cells showed increased transmigration with decreased stiffness. Our findings demonstrate that endothelial monolayers respond to the mechanical properties of their surroundings, which can regulate the integrity and function of the monolayer independently from inflammatory signals. Soft hydrogel substrates are a more appropriate and physiological model for tissue environments than hard substrates, with important implications for the experimental analysis of TEM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.