ArticlePloS one2014
A microfluidic platform for correlative live-cell and super-resolution microscopy.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Integration of secreted signaling molecule sensing on cell monitoring platforms: a critical review.Analytical and bioanalytical chemistry · 2024Review
- PostFocus: automated selective post-acquisition high-throughput focus restoration using diffusion model for label-free time-lapse microscopy.Bioinformatics (Oxford, England) · 2024Article
- Nanotherapeutic Heterogeneity: Sources, Effects, and Solutions.Small (Weinheim an der Bergstrasse, Germany) · 2024Review
- SuperCUT, an unsupervised multimodal image registration with deep learning for biomedical microscopy.Briefings in bioinformatics · 2024Article
- Super-Resolution-Chip: anBiomedical optics express · 2023Article
- Combining multiple fluorescence imaging techniques in biology: when one microscope is not enough.Molecular biology of the cell · 2022Article
- High-resolution imaging of bacterial spatial organization with vertical cell imaging by nanostructured immobilization (VerCINI).Nature protocols · 2022Review
- Can super-resolution microscopy become a standard characterization technique for materials chemistry?Chemical science · 2022Review
- A Review of Optical Imaging Technologies for Microfluidics.Micromachines · 2022Review
- A OneStep Solution to Fix and Stain Cells for Correlative Live and Fixed Microscopy.Current protocols · 2021Article
- Imaging therapeutic peptide transport across intestinal barriers.RSC chemical biology · 2021Review
- Article
- An automated microfluidic device for time-lapse imaging of mouse embryonic stem cells.Biomicrofluidics · 2019Article
- Detyrosinated microtubules spatially constrain lysosomes facilitating lysosome-autophagosome fusion.The Journal of cell biology · 2019Article
- 3D motion of vesicles along microtubules helps them to circumvent obstacles in cells.Journal of cell science · 2017Article
- Shedding light on biology of bacterial cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Organization and dynamics of the actin cytoskeleton during dendritic spine morphological remodeling.Cellular and molecular life sciences : CMLS · 2016Review
- Conventional fluorescence microscopy below the diffraction limit with simultaneous capture of two fluorophores in DNA origami.Proceedings of SPIE--the International Society for Optical Engineering · 2016Article
- Correlated confocal and super-resolution imaging by VividSTORM.Nature protocols · 2016Article
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, super-resolution microscopy methods such as stochastic optical reconstruction microscopy (STORM) have enabled visualization of subcellular structures below the optical resolution limit. Due to the poor temporal resolution, however, these methods have mostly been used to image fixed cells or dynamic processes that evolve on slow time-scales. In particular, fast dynamic processes and their relationship to the underlying ultrastructure or nanoscale protein organization cannot be discerned. To overcome this limitation, we have recently developed a correlative and sequential imaging method that combines live-cell and super-resolution microscopy. This approach adds dynamic background to ultrastructural images providing a new dimension to the interpretation of super-resolution data. However, currently, it suffers from the need to carry out tedious steps of sample preparation manually. To alleviate this problem, we implemented a simple and versatile microfluidic platform that streamlines the sample preparation steps in between live-cell and super-resolution imaging. The platform is based on a microfluidic chip with parallel, miniaturized imaging chambers and an automated fluid-injection device, which delivers a precise amount of a specified reagent to the selected imaging chamber at a specific time within the experiment. We demonstrate that this system can be used for live-cell imaging, automated fixation, and immunostaining of adherent mammalian cells in situ followed by STORM imaging. We further demonstrate an application by correlating mitochondrial dynamics, morphology, and nanoscale mitochondrial protein distribution in live and super-resolution images.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.