Evidence map›Paper›PMID 25544576›Full record

ArticleCancer research and treatment2015

TGF-β Suppresses COX-2 Expression by Tristetraprolin-Mediated RNA Destabilization in A549 Human Lung Cancer Cells.

Soyeong Kang, Ahrum Min, Seock-Ah Im, Sang-Hyun Song, Sang Gyun Kim, Hyun-Ah Kim, Hee-Jun Kim, Do-Youn Oh, Hyun-Soon Jong, Tae-You Kim and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer research and treatment, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Dysregulation of TTP and HuR plays an important role in cancers.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016
    Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Soyeong KangCancer Research Institute, Seoul National University, Seoul, Korea.
Ahrum MinCancer Research Institute, Seoul National University, Seoul, Korea.
Seock-Ah ImCancer Research Institute, Seoul National University, Seoul, Korea ; Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea ; Department of Translational Medicine, Seoul National University College of Medicine, Seoul, Korea.
Sang-Hyun SongCancer Research Institute, Seoul National University, Seoul, Korea.
Sang Gyun KimCancer Research Institute, Seoul National University, Seoul, Korea.
Hyun-Ah KimCancer Research Institute, Seoul National University, Seoul, Korea.
Hee-Jun KimCancer Research Institute, Seoul National University, Seoul, Korea ; Department of Internal Medicine, Chung-Ang University College of Medicine, Seoul, Korea.
Do-Youn OhCancer Research Institute, Seoul National University, Seoul, Korea ; Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
Hyun-Soon JongCancer Research Institute, Seoul National University, Seoul, Korea.
Tae-You KimCancer Research Institute, Seoul National University, Seoul, Korea ; Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea ; Department of Translational Medicine, Seoul National University College of Medicine, Seoul, Korea.
Yung-Jue BangCancer Research Institute, Seoul National University, Seoul, Korea ; Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
Seoul National University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeOverexpression of cyclooxygenase 2 (COX-2) is thought to promote survival of transformed cells. Transforming growth factor β (TGF-β) exerts anti-proliferative effects on a broad range of epithelial cells. In the current study, we investigated whether TGF-β can regulate COX-2 expression in A549 human lung adenocarcinoma cells, which are TGF-β-responsive and overexpress COX-2. MATERIALS AND

methodsWestern blotting, Northern blotting, and mRNA stability assays were performed to demonstrate that COX-2 protein and mRNA expression were suppressed by TGF-β. We also evaluated the effects of tristetraprolin (TTP) on COX-2 mRNA using RNA interference.

resultsWe demonstrated that COX-2 mRNA and protein expression were both significantly suppressed by TGF-β. An actinomycin D chase experiment demonstrated that COX-2 mRNA was more rapidly degraded in the presence of TGF-β, suggesting that TGF-β-induced inhibition of COX-2 expression is achieved via decreased mRNA stability. We also found that TGF-β rapidly and transiently induced the expression of TTP, a well-known mRNA destabilizing factor, before suppression of COX-2 mRNA expression was observed. Using RNA interference, we confirmed that increased TTP levels play a pivotal role in the destabilization of COX-2 mRNA by TGF-β. Furthermore, we showed that Smad3 is essential to TTP-dependent down-regulation of COX-2 expression in response to TGF-β.

conclusionThe results of this study show that TGF-β down-regulated COX-2 expression via mRNA destabilization mediated by Smad3/TTP in A549 cells.

Indexed as

Cyclooxygenase 2RNA stabilitySmad3Transforming growth factor betaTristetraprolin

Identifiers

PMID25544576
PMCPMC4296860
OpenAlexW1994172071

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.