ArticleMolecular cell2015
Adaptive regulation of testis gene expression and control of male fertility by the Drosophila hairpin RNA pathway. [Corrected].
Article in Molecular cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 59 citations in OpenAlex.
- Unraveling circRNAs and ceRNA regulatory networks underlying sex-specific gametogenesis impairment in triploid Pacific oysters (Crassostrea gigas).Biology of sex differences · 2026Article
- Divergent regulatory impacts of endogenous siRNAs on host mRNAs in testis of closely related species.bioRxiv : the preprint server for biology · 2026Article
- Recurrent expansion and rapid evolution of the Drosophilid RNAi pathway in testis.bioRxiv : the preprint server for biology · 2025Article
- Repeated emergence of giant microRNA hairpins across invertebrates.Cell reports · 2025Article
- An innovation in host responses to escalating genomic conflicts.Trends in genetics : TIG · 2025Article
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- Small RNA-mediated suppression of sex chromosome meiotic conflicts during Drosophila male gametogenesis.Biochemical Society transactions · 2025Review
- A low-abundance class of Dicer-dependent siRNAs produced from a variety of features inGenome research · 2024Article
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- New perspectives on the causes and consequences of male meiotic drive.Current opinion in genetics & development · 2023Review
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- Proliferation and dissemination of killer meiotic drive loci.Current opinion in genetics & development · 2023Review
- miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensuresScience advances · 2023Article
- Regulatory logic of endogenous RNAi in silencing de novo genomic conflicts.PLoS genetics · 2023Article
- Essential and recurrent roles for hairpin RNAs in silencing de novo sex chromosome conflict in Drosophila simulans.PLoS biology · 2023Article
- LIRBase: a comprehensive database of long inverted repeats in eukaryotic genomes.Nucleic acids research · 2022Article
- Rapid evolutionary dynamics of an expanding family of meiotic drive factors and their hpRNA suppressors.Nature ecology & evolution · 2021Article
- piRNA-mediated gene regulation and adaptation to sex-specific transposon expression inGenes & development · 2021Article
- A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons.Genome research · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Although endogenous siRNAs (endo-siRNAs) have been described in many species, still little is known about their endogenous utility. Here, we show that Drosophila hairpin RNAs (hpRNAs) generate an endo-siRNA class with predominant expression in testes. Although hpRNAs are universally recently evolved, we identify highly complementary protein-coding targets for all hpRNAs. Importantly, we find broad evidence for evolutionary divergences that preferentially maintain compensatory pairing between hpRNAs and targets, serving as first evidence for adaptive selection for siRNA-mediated target regulation in metazoans. We demonstrate organismal impact of hpRNA activity, since knockout of hpRNA1 derepresses its target ATP synthase-β in testes and compromises spermatogenesis and male fertility. Moreover, we reveal surprising male-specific impact of RNAi factors on germ cell development and fertility, consistent with testis-directed function of the hpRNA pathway. Finally, the collected hpRNA loci chronicle an evolutionary timeline that reflects their origins from prospective target genes, mirroring a strategy described for plant miRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.