ArticleThe Journal of biological chemistry2015
The zebrafish period2 protein positively regulates the circadian clock through mediation of retinoic acid receptor (RAR)-related orphan receptor α (Rorα).
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Brain imaging genetics in ADHD and beyond - Mapping pathways from gene to disorder at different levels of complexity.Neuroscience and biobehavioral reviews · 2017Pooled it
- Selection on PER2 Phosphorylation Regulation Underlies Atypical Mammalian Circadian Rhythms.Ecology and evolution · 2026Article
- CircadianZoological research · 2026Article
- Time-series single-cell transcriptomics reveals pervasive daily rhythmicity and nocturnal spermatogenesis in the zebrafish testis.Science advances · 2026Article
- Circadian Phase Modulates Embryonic Susceptibility to Bisphenol A-Induced ASD-Related Behavioral Alterations viaToxics · 2026Article
- The intricate link between circadian rhythms and aging: can resetting our circadian clock hold the key to longevity?Biogerontology · 2025Review
- Molecular Characterization and Feeding-Associated Expression Dynamics of the Period Gene Family in Channel Catfish (Current issues in molecular biology · 2025Article
- Anti-Müllerian hormone signalling sustains circadian homeostasis in zebrafish.Nature communications · 2025Article
- Direct regulation of Per2 by Roraa: insights into circadian and metabolic interplay in zebrafish.Cellular and molecular life sciences : CMLS · 2025Article
- The circadian clock orchestrates spermatogonial differentiation and fertilization by regulating retinoic acid signaling in vertebrates.National science review · 2025Article
- A miR-219-5p-bmal1b negative feedback loop contributes to circadian regulation in zebrafish.Communications biology · 2024Article
- Thousands of oscillating LncRNAs in the mouse testis.Computational and structural biotechnology journal · 2024Article
- Article
- Contribution of the eye and of opn4xa function to circadian photoentrainment in the diurnal zebrafish.PLoS genetics · 2024Article
- Symptomatic and asymptomatic domoic acid exposure in zebrafish (Danio rerio) revealed distinct non-overlapping gene expression patterns in the brain.Aquatic toxicology (Amsterdam, Netherlands) · 2022Article
- Immunity, Infection, and the Zebrafish Clock.Infection and immunity · 2022Review
- Melanopsin elevates locomotor activity during the wake state of the diurnal zebrafish.EMBO reports · 2022Article
- REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish.International journal of molecular sciences · 2022Article
- Disruption of Circadian Rhythm Genes in Obstructive Sleep Apnea Patients-Possible Mechanisms Involved and Clinical Implication.International journal of molecular sciences · 2022Review
- Shedding light on the circadian clock of the threespine stickleback.The Journal of experimental biology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the characterization of a null mutant for zebrafish circadian clock gene period2 (per2) generated by transcription activator-like effector nuclease and a positive role of PER2 in vertebrate circadian regulation. Locomotor experiments showed that per2 mutant zebrafish display reduced activities under light-dark and 2-h phase delay under constant darkness, and quantitative real time PCR analyses showed up-regulation of cry1aa, cry1ba, cry1bb, and aanat2 but down-regulation of per1b, per3, and bmal1b in per2 mutant zebrafish, suggesting that Per2 is essential for the zebrafish circadian clock. Luciferase reporter assays demonstrated that Per2 represses aanat2 expression through E-box and enhances bmal1b expression through the Ror/Rev-erb response element, implicating that Per2 plays dual roles in the zebrafish circadian clock. Cell transfection and co-immunoprecipitation assays revealed that Per2 enhances bmal1b expression through binding to orphan nuclear receptor Rorα. The enhancing effect of mouse PER2 on Bmal1 transcription is also mediated by RORα even though it binds to REV-ERBα. Moreover, zebrafish Per2 also appears to have tissue-specific regulatory roles in numerous peripheral organs. These findings help define the essential functions of Per2 in the zebrafish circadian clock and in particular provide strong evidence for a positive role of PER2 in the vertebrate circadian system.
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