ArticleAquatic toxicology (Amsterdam, Netherlands)2015
A primary fish gill cell culture model to assess pharmaceutical uptake and efflux: evidence for passive and facilitated transport.
Article in Aquatic toxicology (Amsterdam, Netherlands), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
- Establishment and characterisation of the first cell line from the giant Amazonian fish Arapaima gigas and its susceptibility to Tilapia Lake virus (TiLV).Fish physiology and biochemistry · 2026Article
- Review
- Making in vitro conditions more reflective of in vivo conditions for research on the teleost gastrointestinal tract.The Journal of experimental biology · 2024Review
- Effects of Age and Exposure Duration on the Sensitivity of Early Life Stage Fathead Minnow (Pimephales promelas) to Waterborne Propranolol Exposure.Environmental toxicology and chemistry · 2024Article
- Factors Determining the Susceptibility of Fish to Effects of Human Pharmaceuticals.Environmental science & technology · 2023Review
- Atlantic Salmon Gill Epithelial Cell Line (ASG-10) as a Suitable Model for Xenobiotic Biotransformation.Metabolites · 2023Article
- Minimizing Experimental Testing on Fish for Legacy Pharmaceuticals.Environmental science & technology · 2023Article
- Active Pharmaceutical Ingredient Uptake by Zebrafish (Danio rerio) Oct2 (slc22a2) Transporter Expressed in Xenopus laevis Oocytes.Environmental toxicology and chemistry · 2022Article
- Cultured rainbow trout gill epithelium as anHeliyon · 2021Article
- Marine Fish Primary Hepatocyte Isolation and Culture: New Insights to Enzymatic Dissociation Pancreatin Digestion.International journal of environmental research and public health · 2021Article
- GM1 Oligosaccharide Crosses the Human Blood-Brain Barrier In Vitro by a Paracellular Route.International journal of molecular sciences · 2020Article
- The Use of Molecular Descriptors To Model Pharmaceutical Uptake by a Fish Primary Gill Cell Culture Epithelium.Environmental science & technology · 2019Article
- Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout,Biology open · 2018Article
- Spheroid Size Does not Impact Metabolism of the β-blocker Propranolol in 3D Intestinal Fish Model.Frontiers in pharmacology · 2018Article
- Investigations to extend viability of a rainbow trout primary gill cell culture.Ecotoxicology (London, England) · 2017Article
- Application of the rainbow trout derived intestinal cell line (RTgutGC) for ecotoxicological studies: molecular and cellular responses following exposure to copper.Ecotoxicology (London, England) · 2017Article
- Uptake, biotransformation and elimination of selected pharmaceuticals in a freshwater invertebrate measured using liquid chromatography tandem mass spectrometry.Chemosphere · 2017Article
- Assessing the bioaccumulation potential of ionizable organic compounds: Current knowledge and research priorities.Environmental toxicology and chemistry · 2017Review
- Pharmaceutical Metabolism in Fish: Using a 3-D Hepatic In Vitro Model to Assess Clearance.PloS one · 2017Article
- Assessing the reliability of uptake and elimination kinetics modelling approaches for estimating bioconcentration factors in the freshwater invertebrate, Gammarus pulex.The Science of the total environment · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The gill is the principle site of xenobiotic transfer to and from the aqueous environment. To replace, refine or reduce (3Rs) the large numbers of fish used in in vivo uptake studies an effective in vitro screen is required that mimics the function of the teleost gill. This study uses a rainbow trout (Oncorhynchus mykiss) primary gill cell culture system grown on permeable inserts, which tolerates apical freshwater thus mimicking the intact organ, to assess the uptake and efflux of pharmaceuticals across the gill. Bidirectional transport studies in media of seven pharmaceuticals (propranolol, metoprolol, atenolol, formoterol, terbutaline, ranitidine and imipramine) showed they were transported transcellularly across the epithelium. However, studies conducted in water showed enhanced uptake of propranolol, ranitidine and imipramine. Concentration-equilibrated conditions without a concentration gradient suggested that a proportion of the uptake of propranolol and imipramine is via a carrier-mediated process. Further study using propranolol showed that its transport is pH-dependent and at very low environmentally relevant concentrations (ng L(-1)), transport deviated from linearity. At higher concentrations, passive uptake dominated. Known inhibitors of drug transport proteins; cimetidine, MK571, cyclosporine A and quinidine inhibited propranolol uptake, whilst amantadine and verapamil were without effect. Together this suggests the involvement of specific members of SLC and ABC drug transporter families in pharmaceutical transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.