ArticleMetabolic engineering2015
Dynamic knockdown of E. coli central metabolism for redirecting fluxes of primary metabolites.
Article in Metabolic engineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 167 citations in OpenAlex.
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- A live bacteria enzyme assay for identification of human disease mutations and drug screening.Nature biomedical engineering · 2025Article
- Design principles for engineering bacteria to maximise chemical production from batch cultures.Nature communications · 2025Article
- Cell factories for biosynthesis of D-glucaric acid: a fusion of static and dynamic strategies.World journal of microbiology & biotechnology · 2024Review
- 2-Stage microfermentations.Metabolic engineering communications · 2024Article
- State-of-the-art in engineering small molecule biosensors and their applications in metabolic engineering.SLAS technology · 2024Review
- A self-regulated network for dynamically balancing multiple precursors in complex biosynthetic pathways.Metabolic engineering · 2024Article
- Efficient production of 2'-fucosyllactose from fructose through metabolically engineered recombinant Escherichia coli.Microbial cell factories · 2024Article
- Exploring the potential of myo-inositol in thyroid disease management: focus on thyroid cancer diagnosis and therapy.Frontiers in endocrinology · 2024Review
- Awakening the natural capability of psicose production in Escherichia coli.NPJ science of food · 2023Article
- Dynamic Metabolic Control: From the Perspective of Regulation Logic.Synthetic biology and engineering · 2023Article
- Engineering cell morphology by CRISPR interference in Acinetobacter baylyi ADP1.Microbial biotechnology · 2022Article
- Decoupling Growth and Production by Removing the Origin of Replication from a Bacterial Chromosome.ACS synthetic biology · 2022Article
- Synthetic 3'-UTR valves for optimal metabolic flux control in Escherichia coli.Nucleic acids research · 2022Article
- Harnessing plasmid replication mechanism to enable dynamic control of gene copy in bacteria.Metabolic engineering · 2022Article
- CRISPRi enables fast growth followed by stable aerobic pyruvate formation inEngineering in life sciences · 2022Article
- Removal of lycopene substrate inhibition enables high carotenoid productivity in Yarrowia lipolytica.Nature communications · 2022Article
- E. coli Nissle 1917 modulates host glucose metabolism without directly acting on glucose.Scientific reports · 2021Article
- Dynamic control over feedback regulatory mechanisms improves NADPH flux and xylitol biosynthesis in engineered E. coli.Metabolic engineering · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Control of native enzyme levels is important when optimizing strains for overproduction of heterologous compounds. However, for many central metabolic enzymes, static knockdown results in poor growth and protein expression. We have developed a strategy for dynamically modulating the abundance of native enzymes within the host cell and applied this to a model system for myo-inositol production from glucose. This system relies on controlled degradation of a key glycolytic enzyme, phosphofructokinase-I (Pfk-I). Through tuning Pfk-I levels, we have been able to develop an Escherichia coli strain with a growth mode close to wild type and a production mode with an increased glucose-6-phosphate pool available for conversion into myo-inositol. The switch to production mode is trigged by inducer addition, allowing yield, titer, and productivity to be managed through induction time. By varying the time of Pfk-I degradation, we were able to achieve a two-fold improvement in yield and titers of myo-inositol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.