ArticleFertility and sterility2015
Characterization of primary cultures of adult human epididymis epithelial cells.
Article in Fertility and sterility, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Advancement and Potential Applications of Epididymal Organoids.Biomolecules · 2024Review
- A promoter-dependent upstream activator augments CFTR expression in diverse epithelial cell types.Biochimica et biophysica acta. Gene regulatory mechanisms · 2024Article
- Conditioned medium and secretome from epididymal epithelial cell cultures improve sperm kinetics and capacitation.Veterinary world · 2023Article
- Identification and analysis of differentially expressed (DE) circRNA in epididymis of yak and cattleyak.Frontiers in veterinary science · 2023Article
- Morphological and morphometric changes and epithelial apoptosis are induced in the rat epididymis by long-term letrozole treatment.European journal of histochemistry : EJH · 2021Article
- Trehalose can effectively protect sheep epididymis epithelial cells from oxidative stress.Archives animal breeding · 2021Article
- An atlas of human proximal epididymis reveals cell-specific functions and distinct roles for CFTR.Life science alliance · 2020Article
- An organoid model to assay the role of CFTR in the human epididymis epithelium.Cell and tissue research · 2020Article
- Testosterone promotes GPX5 expression of goat epididymal epithelial cells cultured in vitro.In vitro cellular & developmental biology. Animal · 2019Article
- Transcriptional networks in the human epididymis.Andrology · 2019Review
- Cell-Selective Regulation of CFTR Gene Expression: Relevance to Gene Editing Therapeutics.Genes · 2019Review
- Region-specific innate antiviral responses of the human epididymis.Molecular and cellular endocrinology · 2018Article
- A novel transcriptional network for the androgen receptor in human epididymis epithelial cells.Molecular human reproduction · 2018Article
- A transcription factor network repressesThe Biochemical journal · 2018Article
- Differential contribution of cis-regulatory elements to higher order chromatin structure and expression of the CFTR locus.Nucleic acids research · 2016Article
- HNF1 regulates critical processes in the human epididymis epithelium.Molecular and cellular endocrinology · 2016Article
- Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions.Molecular human reproduction · 2016Article
- Region-specific microRNA signatures in the human epididymis.Asian journal of andrologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveTo establish cultures of epithelial cells from all regions of the human epididymis to provide reagents for molecular approaches to functional studies of this epithelium.
designExperimental laboratory study.
settingUniversity research institute. PATIENT(S): Epididymis from seven patients undergoing orchiectomy for suspected testicular cancer without epididymal involvement. INTERVENTION(S): Human epididymis epithelial cells harvested from adult epididymis tissue. MAIN OUTCOME MEASURE(S): Establishment of a robust culture protocol for adult human epididymal epithelial cells. RESULT(S): Cultures of caput, corpus, and cauda epithelial cells were established from epididymis tissue of seven donors. Cells were passaged up to eight times and maintained differentiation markers. They were also cryopreserved and recovered successfully. Androgen receptor, clusterin, and cysteine-rich secretory protein 1 were expressed in cultured cells, as shown by means of immunofluorescence, Western blot, and quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The distribution of other epididymis markers was also shown by means of qRT-PCR. Cultures developed transepithelial resistance (TER), which was androgen responsive in the caput but androgen insensitive in the corpus and cauda, where unstimulated TER values were much higher. CONCLUSION(S): The results demonstrate a robust in vitro culture system for differentiated epithelial cell types in the caput, corpus, and cauda of the human epididymis. These cells will be a valuable resource for molecular analysis of epididymis epithelial function, which has a pivotal role in male fertility.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.