Evidence map›Paper›PMID 25542802›Full record

ArticleBiomaterials2015

Label-free imaging of gelatin-containing hydrogel scaffolds.

Yajie Liang, Amnon Bar-Shir, Xiaolei Song, Assaf A Gilad, Piotr Walczak, Jeff W M Bulte

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
2.7field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 74 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
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  8. Article
  9. Review
  10. In Vivo Imaging of Implanted Hyaluronic Acid Hydrogel Biodegradation.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  11. Review
  12. Review
  13. Article
  14. Detecting and Monitoring Hydrogels with Medical Imaging.ACS biomaterials science & engineering · 2021
    Review
  15. ACS applied materials & interfaces · 2021
    Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yajie LiangRussell H. Morgan Dept. of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Amnon Bar-ShirRussell H. Morgan Dept. of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Xiaolei SongRussell H. Morgan Dept. of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Assaf A GiladRussell H. Morgan Dept. of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Piotr WalczakRussell H. Morgan Dept. of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Jeff W M BulteRussell H. Morgan Dept. of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Dept. of Chemical & Biomolecular Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Dept. of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Dept. of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: jwmbulte@mri.jhu.edu.
Johns Hopkins University · USJohns Hopkins Medicine · US

Funding

MR Tracking of Magnetically Labeled Stem Cells in CNSR01NS045062 · NINDS · JOHNS HOPKINS UNIVERSITY · PI BULTE, JEFF W. · 2003 to 2013
$4.4M
Efficient Targeting of Therapeutic Cells in Stroke and EAER01NS076573 · NINDS · JOHNS HOPKINS UNIVERSITY · PI WALCZAK, PIOTR · 2012 to 2016
$1.8M
NINDS NIH HHS 2R01 NS045062NINDS NIH HHS R01 NS045062NINDS NIH HHS R01 NS076573
6 · The paper itself

Abstract

Composite hyaluronic acid (HA) hydrogels containing gelatin are used in regenerative medicine as tissue-mimicking scaffolds for improving stem cell survival. Once implanted, it is assumed that these biomaterials disintegrate over time, but at present there is no non-invasive imaging technique available with which such degradation can be directly monitored in vivo. We show here the potential of chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) as a label-free non-invasive imaging technique to monitor dynamic changes in scaffold composition in vivo. The CEST properties of the three individual hydrogel components (HA, GelinS, and polyethylene glycol diacrylate) were first measured in vitro. The complete hydrogel was then injected into the brain of immunodeficient rag2(-/-) mice and CEST MR images were obtained at day 1 and 7 post-transplantation. In vitro, GelinS gave the strongest CEST signal at 3.6 ppm offset from the water peak, originating from the amide protons present in gelatin. In vivo, a significant decrease in CEST signal was observed at 1 week post-implantation. These results were consistent with the biodegradation of the GelinS component, as validated by fluorescent microscopy of implanted hydrogels containing Alexa Fluor 488-labeled GelinS. Our label-free imaging approach should be useful for further development of hydrogel formulations with improved composition and stability.

Indexed as

Imaging, Three-DimensionalStaining and LabelingAnimalsGelatinHydrogel, Polyethylene Glycol DimethacrylateImplants, ExperimentalMagnetic Resonance ImagingMaleMiceTissue ScaffoldsGelatinHydrogel, Polyethylene Glycol DimethacrylateCEST MRIGelatinHyaluronic acidHydrogelScaffoldTransplantation

Identifiers

PMID25542802
PMCPMC4279110
OpenAlexW2091779743

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.