ArticleApplied magnetic resonance2014
Spin-label W-band EPR with seven-loop-six-gap resonator: Application to lens membranes derived from eyes of a single donor.
Article in Applied magnetic resonance, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Spin-Lattice Relaxation Rates of Lipid Spin Labels as a Measure of Their Rotational Diffusion Rates in Lipid Bilayer Membranes.Membranes · 2022Article
- Molecular oxygen as a probe molecule in EPR spin-labeling studies of membrane structure and dynamics.Oxygen (Basel, Switzerland) · 2022Article
- Multilamellar Liposomes as a Model for Biological Membranes: Saturation Recovery EPR Spin-Labeling Studies.Membranes · 2022Review
- Oxygen Transport Parameter in Plasma Membrane of Eye Lens Fiber Cells by Saturation Recovery EPR.Applied magnetic resonance · 2021Article
- Uniform Field Resonators for EPR Spectroscopy: A Review.Cell biochemistry and biophysics · 2019Review
- An x-band continuous wave saturation recovery electron paramagnetic resonance spectrometer based on an arbitrary waveform generator.The Review of scientific instruments · 2019Article
- Saturation recovery EPR spin-labeling method for quantification of lipids in biological membrane domains.Applied magnetic resonance · 2017Article
- Autobiography of James S. Hyde.Applied magnetic resonance · 2017Article
- EPR UNIFORM FIELD SIGNAL ENHANCEMENT BY DIELECTRIC TUBES IN CAVITIES.Applied magnetic resonance · 2017Article
- Broadband W-band Rapid Frequency Sweep Considerations for Fourier Transform EPR.Cell biochemistry and biophysics · 2017Article
- Uniform field loop-gap resonator and rectangular TEJournal of magnetic resonance (San Diego, Calif. : 1997) · 2017Article
- Spin-labeled small unilamellar vesicles with theApplied magnetic resonance · 2015Article
- Properties of membranes derived from the total lipids extracted from clear and cataractous lenses of 61-70-year-old human donors.European biophysics journal : EBJ · 2015Article
- Uniform Field Re-entrant Cylindrical TE[Formula: see text] Cavity for Pulse Electron Paramagnetic Resonance Spectroscopy at Q-band.Applied magnetic resonanceArticle
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
Spin-label W-band (94 GHz) EPR with a five-loop-four-gap resonator (LGR) was successfully applied to study membrane properties (L. Mainali, J.S. Hyde, W.K. Subczynski, Using spin-label W-band EPR to study membrane fluidity in samples of small volume, J. Magn. Reson. 226 (2013) 35-44). In that study, samples were equilibrated with the selected gas mixture outside the resonator in a sample volume ~100 times larger than the sensitive volume of the LGR and transferred to the resonator in a quartz capillary. A seven-loop-six-gap W-band resonator has been developed. This resonator permits measurements on aqueous samples of 150 nL volume positioned in a polytetrafluoroethylene (PTFE) gas permeable sample tube. Samples can be promptly deoxygenated or equilibrated with an air/nitrogen mixture inside the resonator, which is significant in saturation-recovery measurements and in spin-label oximetry. This approach was tested for lens lipid membranes derived from lipids extracted from two porcine lenses (single donor). Profiles of membrane fluidity and the oxygen transport parameter were obtained from saturation-recovery EPR using phospholipid analog spin-labels. Cholesterol analog spin-labels allowed discrimination of the cholesterol bilayer domain and acquisition of oxygen transport parameter profiles across this domain. Results were compared with those obtained previously for membranes derived from a pool of 100 lenses. Results demonstrate that EPR at W-band can be successfully used to study aqueous biological samples of small volume under controlled oxygen concentration.
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