ArticleBiochemistry2015
Dynamic architecture of the purinosome involved in human de novo purine biosynthesis.
Article in Biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 50 citations in OpenAlex.
- Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.Advances in experimental medicine and biology · 2026Review
- Localising enzymes to biomolecular condensates increases their accumulation and benefits engineered metabolic pathway performance in Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- Mechanistic insights into condensate formation of human liver-type phosphofructokinase by stochastic modeling approaches.Scientific reports · 2024Article
- Chaperone regulation of biomolecular condensates.Frontiers in biophysics · 2024Article
- Purinosomes involved in the regulation of tumor metabolism: current progress and potential application targets.Frontiers in oncology · 2024Review
- Size-Specific Modulation of a Multienzyme Glucosome Assembly during the Cell Cycle.ACS bio & med chem Au · 2023Article
- The roles of molecular chaperones in regulating cell metabolism.FEBS letters · 2023Review
- De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence.Nature cell biology · 2023Article
- The Purinosome: A Case Study for a Mammalian Metabolon.Annual review of biochemistry · 2022Review
- Purine biosynthetic enzymes assemble into liquid-like condensates dependent on the activity of chaperone protein HSP90.The Journal of biological chemistry · 2022Article
- Liquid-liquid phase separation drives cellular function and dysfunction in cancer.Nature reviews. Cancer · 2022Review
- Identification of molecular subtypes based on liquid-liquid phase separation and cross-talk with immunological phenotype in bladder cancer.Frontiers in immunology · 2022Article
- Multi-dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Liquid-liquid phase separation: a principal organizer of the cell's biochemical activity architecture.Trends in pharmacological sciences · 2021Review
- Possible Therapeutic Strategy Involving the Purine Synthesis Pathway Regulated by ITK in Tongue Squamous Cell Carcinoma.Cancers · 2021Article
- Human de novo purine biosynthesis.Critical reviews in biochemistry and molecular biology · 2021Review
- Hypoxia drives the assembly of the multienzyme purinosome complex.The Journal of biological chemistry · 2020Article
- Metabolomics and mass spectrometry imaging reveal channeled de novo purine synthesis in cells.Science (New York, N.Y.) · 2020Article
- Breaking the Fourth Wall: Modulating Quaternary Associations for Protein Regulation and Drug Discovery.Chembiochem : a European journal of chemical biology · 2019Review
- Phase-separated condensates of metabolic complexes in living cells: Purinosome and glucosome.Methods in enzymology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Enzymes in human de novo purine biosynthesis have been demonstrated to form a reversible, transient multienzyme complex, the purinosome, upon purine starvation. However, characterization of purinosomes has been limited to HeLa cells and has heavily relied on qualitative examination of their subcellular localization and reversibility under wide-field fluorescence microscopy. Quantitative approaches, which are particularly compatible with human disease-relevant cell lines, are necessary to explicitly understand the purinosome in live cells. In this work, human breast carcinoma Hs578T cells have been utilized to demonstrate the preferential utilization of the purinosome under purine-depleted conditions. In addition, we have employed a confocal microscopy-based biophysical technique, fluorescence recovery after photobleaching, to characterize kinetic properties of the purinosome in live Hs578T cells. Quantitative characterization of the diffusion coefficients of all de novo purine biosynthetic enzymes reveals the significant reduction of their mobile kinetics upon purinosome formation, the dynamic partitioning of each enzyme into the purinosome, and the existence of three intermediate species in purinosome assembly under purine starvation. We also demonstrate that the diffusion coefficient of the purine salvage enzyme, hypoxanthine phosphoribosyltransferase 1, is not sensitive to purine starvation, indicating exclusion of the salvage pathway from the purinosome. Furthermore, our biophysical characterization of nonmetabolic enzymes clarifies that purinosomes are spatiotemporally different cellular bodies from stress granules and cytoplasmic protein aggregates in both Hs578T and HeLa cells. Collectively, quantitative analyses of the purinosome in Hs578T cells led us to provide novel insights for the dynamic architecture of the purinosome assembly.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.