Evidence map›Paper›PMID 25540400›Full record

ArticleJournal of clinical microbiology2015

Global multilocus sequence typing analysis of Mycoplasma bovis isolates reveals two main population clusters.

R S Rosales, C P Churchward, C Schnee, K Sachse, I Lysnyansky, S Catania, L Iob, R D Ayling, R A J Nicholas

Erratum issuedOpen access · bronzeAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
3.3field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 50 citations in OpenAlex.

  1. Article
  2. Article
  3. Genetic structure and diversity ofFrontiers in veterinary science · 2024
    Article
  4. Pathogenicity and molecular characterization of Mycoplasma bovis isolate from calves in Morocco.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023
    Review
  5. Article
  6. Article
  7. Article
  8. Pathogens (Basel, Switzerland) · 2020
    Article
  9. Population Genomic Analysis ofMicroorganisms · 2020
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. A review of mycoplasma diagnostics in cattle.Journal of veterinary internal medicine · 2018
    Review
  18. Multi-locus sequence types of Mycoplasma bovis isolated from Ontario, Canada in the past three decades have a temporal distribution.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2018
    Article
  19. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 4 countries.

R S RosalesAnimal and Plant Health Agency-Weybridge, Addlestone, Surrey, United Kingdom ruben.rosales@apha.gsi.gov.uk.
C P ChurchwardAnimal and Plant Health Agency-Weybridge, Addlestone, Surrey, United Kingdom.
C SchneeInstitute for Molecular Pathogenesis, Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Jena, Germany.
K SachseInstitute for Molecular Pathogenesis, Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Jena, Germany.
I LysnyanskyKimron Veterinary Institute, Beit Dagan, Israel.
S CataniaIstituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy.
L IobIstituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy.
R D AylingAnimal and Plant Health Agency-Weybridge, Addlestone, Surrey, United Kingdom.
R A J NicholasAnimal and Plant Health Agency-Weybridge, Addlestone, Surrey, United Kingdom.
Animal and Plant Health Agency · GBFriedrich-Loeffler-Institut · DEIstituto Zooprofilattico Sperimentale delle Venezie · ITMinistry of Agriculture and Rural Development · IL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycoplasma bovis is a major bovine pathogen associated with bovine respiratory disease complex and is responsible for substantial economic losses worldwide. M. bovis is also associated with other clinical presentations in cattle, including mastitis, otitis, arthritis, and reproductive disorders. To gain a better understanding of the genetic diversity of this pathogen, a multilocus sequence typing (MLST) scheme was developed and applied to the characterization of 137 M. bovis isolates from diverse geographical origins, obtained from healthy or clinically infected cattle. After in silico analysis, a final set of 7 housekeeping genes was selected (dnaA, metS, recA, tufA, atpA, rpoD, and tkt). MLST analysis demonstrated the presence of 35 different sequence types (STs) distributed in two main clonal complexes (CCs), defined at the double-locus variant level, namely, CC1, which included most of the British and German isolates, and CC2, which was a more heterogeneous and geographically distant group of isolates, including European, Asian, and Australian samples. Index of association analysis confirmed the clonal nature of the investigated M. bovis population, based on MLST data. This scheme has demonstrated high discriminatory power, with the analysis showing the presence of genetically distant and divergent clusters of isolates predominantly associated with geographical origins.

Indexed as

Cluster AnalysisGenetic VariationMultilocus Sequence TypingAnimalsCattleCattle DiseasesGenes, BacterialGenes, EssentialGenotypeGlobal HealthMolecular EpidemiologyMolecular Sequence DataMycoplasma bovisMycoplasma InfectionsPhylogeography

Identifiers

PMID25540400
PMCPMC4390661
OpenAlexW2035761061

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.