ArticleClinical and vaccine immunology : CVI2015
Immunization with Brucella VirB proteins reduces organ colonization in mice through a Th1-type immune response and elicits a similar immune response in dogs.
Article in Clinical and vaccine immunology : CVI, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 2 of them syntheses that pooled it.
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Who cites it
17 citing papers in PubMed, 2 syntheses or guidelines pooled it, 22 citations in OpenAlex.
- Meta-Analysis of the Changes of Peripheral Blood T Cell Subsets in Patients with Brucellosis.Journal of immunology research · 2018Pooled it
- Meta-Analysis and Advancement of Brucellosis Vaccinology.PloS one · 2016Pooled it
- A Putative Multicopper Oxidase Encoded byInternational journal of molecular sciences · 2026Article
- ComparativeVeterinary world · 2026Article
- In Silico Design and Characterization of a Multiepitope Vaccine Candidate AgainstJournal of immunology research · 2025Article
- Preparation and evaluation ofFrontiers in cellular and infection microbiology · 2024Article
- Key immunity characteristics of diverse stages of brucellosis in rural population from Inner Mongolia, China.Infectious diseases of poverty · 2022Article
- Protection against virulentClinical and experimental vaccine research · 2022Article
- Secretory System Components as Potential Prophylactic Targets for Bacterial Pathogens.Biomolecules · 2021Review
- Canine Brucellosis: An Update.Frontiers in veterinary science · 2021Review
- Prevalence and Genomic Characterization ofAnimals : an open access journal from MDPI · 2020Article
- Article
- Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs.PloS one · 2020Article
- Characterization of Brucella canis infection in mice.PloS one · 2019Article
- Review
- Recombinant Enolase ofJournal of immunology research · 2018Article
- Preliminary study on the role of novel LysR family gene kp05372 in Klebsiella pneumoniae of forest musk deer.Journal of Zhejiang University. Science. BArticle
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
VirB proteins from Brucella spp. constitute the type IV secretion system, a key virulence factor mediating the intracellular survival of these bacteria. Here, we assessed whether a Th1-type immune response against VirB proteins may protect mice from Brucella infection and whether this response can be induced in the dog, a natural host for Brucella. Splenocytes from mice immunized with VirB7 or VirB9 responded to their respective antigens with significant and specific production of gamma interferon (IFN-γ), whereas interleukin-4 (IL-4) was not detected. Thirty days after an intraperitoneal challenge with live Brucella abortus, the spleen load of bacteria was almost 1 log lower in mice immunized with VirB proteins than in unvaccinated animals. As colonization reduction seemed to correlate with a Th1-type immune response against VirB proteins, we decided to assess whether such a response could be elicited in the dog. Peripheral blood mononuclear cells (PBMCs) from dogs immunized with VirB proteins (three subcutaneous doses in QuilA adjuvant) produced significantly higher levels of IFN-γ than cells from control animals upon in vitro stimulation with VirB proteins. A skin test to assess specific delayed-type hypersensitivity was positive in 4 out of 5 dogs immunized with either VirB7 or VirB9. As both proteins are predicted to locate in the outer membrane of Brucella organisms, the ability of anti-VirB antibodies to mediate complement-dependent bacteriolysis of B. canis was assessed in vitro. Sera from dogs immunized with either VirB7 or VirB9, but not from those receiving phosphate-buffered saline (PBS), produced significant bacteriolysis. These results suggest that VirB-specific responses that reduce organ colonization by Brucella in mice can be also elicited in dogs.
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