ArticleJournal of industrial microbiology & biotechnology2015
Deletion of genes involved in glutamate metabolism to improve poly-gamma-glutamic acid production in B. amyloliquefaciens LL3.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Poly (γ) glutamic acid: a unique microbial biopolymer with diverse commercial applicability.Frontiers in microbiology · 2024Review
- Effects of FeMicrobial cell factories · 2023Article
- Poly-gamma-glutamic acid biopolymer: a sleeping giant with diverse applications and unique opportunities for commercialization.Biomass conversion and biorefinery · 2023Review
- Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid.Microbial cell factories · 2022Article
- Genetic and metabolic engineering for poly-γ-glutamic acid production: current progress, challenges, and prospects.World journal of microbiology & biotechnology · 2022Review
- γ-Polyglutamic Acid Production, Biocontrol, and Stress Tolerance: Multifunction ofInternational journal of environmental research and public health · 2022Article
- Recent Advances in Microbial Synthesis of Poly-γ-Glutamic Acid: A Review.Foods (Basel, Switzerland) · 2022Review
- Metabolic engineering of Bacillus amyloliquefaciens LL3 for enhanced poly-γ-glutamic acid synthesis.Microbial biotechnology · 2019Article
- Article
- Chungkookjang with High Contents of Poly-γ-Glutamic Acid Improves Insulin Sensitizing Activity in Adipocytes and Neuronal Cells.Nutrients · 2018Article
- Poly-γ-glutamic Acid Synthesis, Gene Regulation, Phylogenetic Relationships, and Role in Fermentation.International journal of molecular sciences · 2017Review
- A novel approach to improve poly-γ-glutamic acid production by NADPH Regeneration in Bacillus licheniformis WX-02.Scientific reports · 2017Article
- Improvement of Bacillus subtilis for poly-γ-glutamic acid production by genome shuffling.Microbial biotechnology · 2016Article
- Microbial synthesis of poly-γ-glutamic acid: current progress, challenges, and future perspectives.Biotechnology for biofuels · 2016Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we attempted to elevate poly-gamma-glutamic acid (γ-PGA) production by modifying genes involved in glutamate metabolism in Bacillus amyloliquefaciens LL3. Products of rocR, rocG and gudB facilitate the conversion from glutamate to 2-oxoglutarate in Bacillus subtillis. The gene odhA is responsible for the synthesis of a component of the 2-oxoglutarate dehydrogenase complex that catalyzes the oxidative decarboxylation of 2-oxoglutarate to succinyl coenzyme A. In-frame deletions of these four genes were performed. In shake flask experiments the gudB/rocG double mutant presented enhanced production of γ-PGA, a 38 % increase compared with wild type. When fermented in a 5-L fermenter with pH control, the γ-PGA yield of the rocR mutant was increased to 5.83 g/L from 4.55 g/L for shake flask experiments. The gudB/rocG double mutant produced 5.68 g/L γ-PGA compared with that of 4.03 g/L for the wild type, a 40 % increase. Those results indicated the possibility of improving γ-PGA production by modifying glutamate metabolism, and identified potential genetic targets to improve γ-PGA production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.