ArticleNucleic acids research2015
Tumor-associated mutations in a conserved structural motif alter physical and biochemical properties of human RAD51 recombinase.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Molecular signaling network and therapeutic developments in breast cancer brain metastasis.Advances in cancer biology - metastasis · 2023Article
- RAD51 bypasses the CMG helicase to promote replication fork reversal.Science (New York, N.Y.) · 2023Article
- A Pan-Cancer Bioinformatic Analysis of RAD51 Regarding the Values for Diagnosis, Prognosis, and Therapeutic Prediction.Frontiers in oncology · 2022Article
- Homologous Recombination Deficiency: Cancer Predispositions and Treatment Implications.The oncologist · 2021Review
- Article
- MicroRNA-107 promotes apoptosis of acute myelocytic leukemia cells by targeting RAD51.Archives of medical science : AMS · 2021Article
- Spatial and temporal organization of RecA in theeLife · 2019Article
- Electrophilic fatty acids impair RAD51 function and potentiate the effects of DNA-damaging agents on growth of triple-negative breast cells.The Journal of biological chemistry · 2019Article
- Discovery of mutations in homologous recombination genes in African-American women with breast cancer.Familial cancer · 2018Article
- Variation in RAD51 details a hub of functions: opportunities to advance cancer diagnosis and therapy.F1000Research · 2018Review
- Defects in recombination activity caused by somatic and germline mutations in the multimerization/BRCA2 binding region of human RAD51 protein.DNA repair · 2017Article
- A cell-penetrating antibody inhibits human RAD51 via direct binding.Nucleic acids research · 2017Article
- High-resolution structure of the presynaptic RAD51 filament on single-stranded DNA by electron cryo-microscopy.Nucleic acids research · 2016Article
- The Tumor-Associated Variant RAD51 G151D Induces a Hyper-Recombination Phenotype.PLoS genetics · 2016Article
- RAD51 variant proteins from human lung and kidney tumors exhibit DNA strand exchange defects.DNA repair · 2016Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Human RAD51 protein catalyzes DNA pairing and strand exchange reactions that are central to homologous recombination and homology-directed DNA repair. Successful recombination/repair requires the formation of a presynaptic filament of RAD51 on ssDNA. Mutations in BRCA2 and other proteins that control RAD51 activity are associated with human cancer. Here we describe a set of mutations associated with human breast tumors that occur in a common structural motif of RAD51. Tumor-associated D149N, R150Q and G151D mutations map to a Schellman loop motif located on the surface of the RecA homology domain of RAD51. All three variants are proficient in DNA strand exchange, but G151D is slightly more sensitive to salt than wild-type (WT). Both G151D and R150Q exhibit markedly lower catalytic efficiency for adenosine triphosphate hydrolysis compared to WT. All three mutations alter the physical properties of RAD51 nucleoprotein filaments, with G151D showing the most dramatic changes. G151D forms mixed nucleoprotein filaments with WT RAD51 that have intermediate properties compared to unmixed filaments. These findings raise the possibility that mutations in RAD51 itself may contribute to genome instability in tumor cells, either directly through changes in recombinase properties, or indirectly through changes in interactions with regulatory proteins.
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