ArticleBMC microbiology2014
Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 21 citations in OpenAlex.
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- Natural Transformation in a Classical-Biotype Vibrio cholerae Strain.Applied and environmental microbiology · 2021Article
- Natural Transformation inFrontiers in microbiology · 2021Article
- Incompatibility of Vibrio fischeri Strains during Symbiosis Establishment Depends on Two Functionally RedundantJournal of bacteriology · 2019Article
- A Halocin Promotes DNA Uptake inFrontiers in microbiology · 2019Article
- QstR-dependent regulation of natural competence and type VI secretion in Vibrio cholerae.Nucleic acids research · 2018Article
- Glucose-Specific Enzyme IIA of the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Modulates Chitin Signaling Pathways in Vibrio cholerae.Journal of bacteriology · 2017Article
- Rules of Engagement: The Type VI Secretion System in Vibrio cholerae.Trends in microbiology · 2017Review
- Effects of Carbohydrate Source on Genetic Competence in Streptococcus mutans.Applied and environmental microbiology · 2016Article
- CytR Is a Global Positive Regulator of Competence, Type VI Secretion, and Chitinases in Vibrio cholerae.PloS one · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe human pathogen Vibrio cholerae normally enters the developmental program of natural competence for transformation after colonizing chitinous surfaces. Natural competence is regulated by at least three pathways in this organism: chitin sensing/degradation, quorum sensing and carbon catabolite repression (CCR). The cyclic adenosine monophosphate (cAMP) receptor protein CRP, which is the global regulator of CCR, binds to regulatory DNA elements called CRP sites when in complex with cAMP. Previous studies in Haemophilus influenzae suggested that the CRP protein binds competence-specific CRP-S sites under competence-inducing conditions, most likely in concert with the master regulator of transformation Sxy/TfoX.
resultsIn this study, we investigated the regulation of the competence genes qstR and comEA as an example of the complex process that controls competence gene activation in V. cholerae. We identified previously unrecognized putative CRP-S sites upstream of both genes. Deletion of these motifs significantly impaired natural transformability. Moreover, site-directed mutagenesis of these sites resulted in altered gene expression. This altered gene expression also correlated directly with protein levels, bacterial capacity for DNA uptake, and natural transformability.
conclusionsBased on the data provided in this study we suggest that the identified sites are important for the expression of the competence genes qstR and comEA and therefore for natural transformability of V. cholerae even though the motifs might not reflect bona fide CRP-S sites.
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