ArticleBiomicrofluidics2014
An on-chip study on the influence of geometrical confinement and chemical gradient on cell polarity.
Article in Biomicrofluidics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Cytotoxic responses of carnosic acid and doxorubicin on breast cancer cells in butterfly-shaped microchips in comparison to 2D and 3D culture.Cytotechnology · 2017Article
- Preface to Special Topic: Selected Papers from the Advances in Microfluidics and Nanofluidics 2014 Conference in Honor of Professor Hsueh-Chia Chang's 60th Birthday.Biomicrofluidics · 2014Article
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Authors and funding
8 authors.
Funding
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Abstract
Cell polarity plays key roles in tissue development, regeneration, and pathological processes. However, how the cells establish and maintain polarity is still obscure so far. In this study, by employing microfluidic techniques, we explored the influence of geometrical confinement and chemical stimulation on the cell polarity and their interplay. We found that teardrop shape-induced anterior/posterior polarization of cells displayed homogeneous distribution of epidermal growth factor receptor, and the polarity could be maintained in a uniform epidermal growth factor (EGF) solution, but be broken by a reverse gradient of EGF, implying different mechanism of geometrical and chemical cue-induced cell polarity. Further studies indicated that a teardrop pattern could cause polarized distribution of microtubule-organization center and nucleus-Golgi complex, and this polarity was weakened when the cells were released from the confinement. Our study provides the evidence regarding the difference between geometrical and chemical cue-induced cell polarity and would be useful for understanding relationship between polarity and directional migration of cells.
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Registered trials
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