Evidence map›Paper›PMID 25538746›Full record

ArticleNeural regeneration research2012

Use of hybrid chitosan membranes and human mesenchymal stem cells from the Wharton jelly of umbilical cord for promoting nerve regeneration in an axonotmesis rat model.

Andrea Gärtner, Tiago Pereira, Maria João Simões, Paulo As Armada-da-Silva, Miguel L França, Rosa Sousa, Simone Bompasso, Stefania Raimondo, Yuki Shirosaki, Yuri Nakamura and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neural regeneration research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Review
  2. Fibrin Glue Coating Limits Scar Tissue Formation around Peripheral Nerves.International journal of molecular sciences · 2024
    Article
  3. Review
  4. Review
  5. Article
  6. Review
  7. Article
  8. Dental pulp stem cells and BonelikeRegenerative biomaterials · 2019
    Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Andrea GärtnerAnimal Science and Study Centre / Food and Agrarian Sciences and Technologies Institute, Porto University, 4099-003 Porto, Portugal ; Institute of Biomedical Sciences Abel Salazar, Veterinary Clinics Department, Porto University, Porto 4099-003, Portugal.
Tiago PereiraAnimal Science and Study Centre / Food and Agrarian Sciences and Technologies Institute, Porto University, 4099-003 Porto, Portugal ; Institute of Biomedical Sciences Abel Salazar, Veterinary Clinics Department, Porto University, Porto 4099-003, Portugal.
Maria João SimõesAnimal Science and Study Centre / Food and Agrarian Sciences and Technologies Institute, Porto University, 4099-003 Porto, Portugal ; Institute of Biomedical Sciences Abel Salazar, Veterinary Clinics Department, Porto University, Porto 4099-003, Portugal.
Paulo As Armada-da-SilvaFaculty of Human Kinetics, Technical University of Lisbon, Cruz Quebrada - Dafundo, 1499-002, Portugal.
Miguel L FrançaAnimal Science and Study Centre / Food and Agrarian Sciences and Technologies Institute, Porto University, 4099-003 Porto, Portugal ; Institute of Biomedical Sciences Abel Salazar, Veterinary Clinics Department, Porto University, Porto 4099-003, Portugal.
Rosa SousaInstitute of Biomedical Sciences Abel Salazar, Cytogenetic Department, Porto University, Porto 4099-003, Portugal.
Simone BompassoNeuroscience Institute of the Cavalieri Ottolenghi Foundation, Orbassano 10043, Turin, Italy ; Department of Clinical and Biological Sciences, University of Turin, Orbassano 10010, Turin, Italy.
Stefania RaimondoNeuroscience Institute of the Cavalieri Ottolenghi Foundation, Orbassano 10043, Turin, Italy ; Department of Clinical and Biological Sciences, University of Turin, Orbassano 10010, Turin, Italy.
Yuki ShirosakiGraduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
Yuri NakamuraFaculty of Engineering, Okayama University, Okayama 700-8530, Japan.
Satoshi HayakawaGraduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
Akiyoshi OsakahGraduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
Beatriz PortoInstitute of Biomedical Sciences Abel Salazar, Cytogenetic Department, Porto University, Porto 4099-003, Portugal.
Ana Lúcia LuísAnimal Science and Study Centre / Food and Agrarian Sciences and Technologies Institute, Porto University, 4099-003 Porto, Portugal ; Institute of Biomedical Sciences Abel Salazar, Veterinary Clinics Department, Porto University, Porto 4099-003, Portugal.
Artur Sp VarejãoDepartment of Veterinary Sciences, Research Centre in Sports, Health and Human Development, University of Trás-os-Montes and Alto Douro, Vila Real 5001-801, Portugal.
Ana Colette MaurícioAnimal Science and Study Centre / Food and Agrarian Sciences and Technologies Institute, Porto University, 4099-003 Porto, Portugal ; Institute of Biomedical Sciences Abel Salazar, Veterinary Clinics Department, Porto University, Porto 4099-003, Portugal.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many studies have been dedicated to the development of scaffolds for improving post-traumatic nerve regeneration. The goal of this study was to assess the effect on nerve regeneration, associating a hybrid chitosan membrane with non-differentiated human mesenchymal stem cells isolated from Wharton's jelly of umbilical cord, in peripheral nerve reconstruction after crush injury. Chromosome analysis on human mesenchymal stem cell line from Wharton's jelly was carried out and no structural alterations were found in metaphase. Chitosan membranes were previously tested in vitro, to assess their ability in supporting human mesenchymal stem cell survival, expansion, and differentiation. For the in vivo testing, Sasco Sprague adult rats were divided in 4 groups of 6 or 7 animals each: Group 1, sciatic axonotmesis injury without any other intervention (Group 1-Crush); Group 2, the axonotmesis lesion of 3 mm was infiltrated with a suspension of 1 250-1 500 human mesenchymal stem cells (total volume of 50 μL) (Group 2-CrushCell); Group 3, axonotmesis lesion of 3 mm was enwrapped with a chitosan type III membrane covered with a monolayer of non-differentiated human mesenchymal stem cells (Group 3-CrushChitIIICell) and Group 4, axonotmesis lesion of 3 mm was enwrapped with a chitosan type III membrane (Group 4-CrushChitIII). Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks using sciatic functional index, static sciatic index, extensor postural thrust, and withdrawal reflex latency. Stereological analysis was carried out on regenerated nerve fibers. Results showed that infiltration of human mesenchymal stem cells, or the combination of chitosan membrane enwrapment and human mesenchymal stem cell enrichment after nerve crush injury provide a slight advantage to post-traumatic nerve regeneration. Results obtained with chitosan type III membrane alone confirmed that they significantly improve post-traumatic axonal regrowth and may represent a very promising clinical tool in peripheral nerve reconstructive surgery. Yet, umbilical cord human mesenchymal stem cells, that can be expanded in culture and induced to form several different types of cells, may prove, in future experiments, to be a new source of cells for cell therapy, including targets such as peripheral nerve and muscle.

Indexed as

axonotmesisbiomaterialschitosanfunctional analysiskaryotype analysismesenchymal stem cellsratstem cellsstereological analysisumbilical cordWharton jelly

Identifiers

PMID25538746
PMCPMC4268725

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.