ArticleFrontiers in plant science2014
Cellular dynamics during early barley pollen embryogenesis revealed by time-lapse imaging.
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- In Vitro Live Cell Imaging Reveals Nuclear Dynamics and Role of the Cytoskeleton During Asymmetric Division of Pollen Mitosis I in Nicotiana Benthamiana.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Visualizing Pollen and Pollen Tube Development Using Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Molecular Insights into Microspore Embryogenesis: Transcriptomic Dynamics in Barley and Wheat.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Establishment and maintenance of embryogenic cell fate during microspore embryogenesis.The Plant journal : for cell and molecular biology · 2025Article
- Transcriptomic identification of potential antioxidative enzyme regulators of the gametophytic-to-embryogenic switch in barley microspores.Frontiers in plant science · 2025Article
- Microtubule organization changes severely after mannitol and n-butanol treatments inducing microspore embryogenesis in bread wheat.BMC plant biology · 2021Article
- Live Imaging of embryogenic structures in Brassica napus microspore embryo cultures highlights the developmental plasticity of induced totipotent cells.Plant reproduction · 2020Article
- Trichostatin A Affects Developmental Reprogramming of Bread Wheat Microspores towards an Embryogenic Route.Plants (Basel, Switzerland) · 2020Article
- In vitro androgenesis: spontaneous vs. artificial genome doubling and characterization of regenerants.Plant cell reports · 2020Review
- The Effect of Caffeine and Trifluralin on Chromosome Doubling in Wheat Anther Culture.Plants (Basel, Switzerland) · 2020Article
- Effect of Zearalenone and Hormone Regulators on Microspore Embryogenesis in Anther Culture of Wheat.Plants (Basel, Switzerland) · 2019Article
- Cas Endonuclease Technology-A Quantum Leap in the Advancement of Barley and Wheat Genetic Engineering.International journal of molecular sciences · 2019Review
- Dynamics of post-translationally modified histones during barley pollen embryogenesis in the presence or absence of the epi-drug trichostatin A.Plant reproduction · 2017Article
- The low molecular weight fraction of compounds released from immature wheat pistils supports barley pollen embryogenesis.Frontiers in plant science · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants display a remarkable capacity for cellular totipotency. An intriguing and useful example is that immature pollen cultured in vitro can pass through embryogenic development to form haploid or doubled haploid plants. However, a lack of understanding the initial mechanisms of pollen embryogenesis hampers the improvement and more effective and widespread employment of haploid technology in plant research and breeding. To investigate the cellular dynamics during the onset of pollen embryogenesis, we used time-lapse imaging along with transgenic barley expressing nuclear localized Green Fluorescent Protein. The results enabled us to identify nine distinct embryogenic and non-embryogenic types of pollen response to the culture conditions. Cell proliferation in embryogenic pollen normally started via a first symmetric mitosis (54.3% of pollen observed) and only rarely did so via asymmetric pollen mitosis I (4.3% of pollen observed). In the latter case, proliferation generally originated from the vegetative-like cell, albeit the division of the generative-like cell was observed in few types of pollen. Under the culture conditions used, fusion of cell nuclei was the only mechanism of genome duplication observed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.