ArticleThe Plant cell2014
The Arabidopsis abiotic stress-induced TSPO-related protein reduces cell-surface expression of the aquaporin PIP2;7 through protein-protein interactions and autophagic degradation.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 152 citations in OpenAlex.
- Maize ZmbZIP92 transcription factor positively regulates drought tolerance inPlant signaling & behavior · 2026Article
- Transcriptional and post-translational regulation of aquaporins in plants.Stress biology · 2026Review
- Autophagy-phytohormone crosstalk: a dual-regulation axis in plant development.Frontiers in plant science · 2026Review
- Dehydrin Protein TaCOR410 Improves Drought Resistance of Wheat Through Autophagy.Plants (Basel, Switzerland) · 2025Article
- Plasma membrane CYBDOM proteins catalyse apoplastic AsA regeneration and interact with RbohD to activate autophagy and drought tolerance in plants.Nature plants · 2025Article
- An elite allele TaDT1-ANature communications · 2025Article
- Phosphorylation of PIP2;7 by CPK28 or Phytophthora kinase effectors dampens pattern-triggered immunity in Arabidopsis.Plant communications · 2025Article
- The lowdown on breakdown: Open questions in plant proteolysis.The Plant cell · 2024Review
- Water fluxes pattern growth and identity in shoot meristems.Nature communications · 2024Article
- NBR1-mediated selective autophagy of ARF7 modulates root branching.EMBO reports · 2024Article
- The Role of Plant Ubiquitin-like Modifiers in the Formation of Salt Stress Tolerance.Plants (Basel, Switzerland) · 2024Review
- Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress.Journal of experimental botany · 2023Article
- Autophagy and multivesicular body pathways cooperate to protect sulfur assimilation and chloroplast functions.Plant physiology · 2023Article
- Salt Stress-Regulation of Root Water Uptake in a Whole-Plant and Diurnal Context.International journal of molecular sciences · 2023Review
- New TSPO Crystal Structures of Mutant and Heme-Bound Forms with Altered Flexibility, Ligand Binding, and Porphyrin Degradation Activity.Biochemistry · 2023Article
- Review
- Ubiquitin ligase OsRINGzf1 regulates drought resistance by controlling the turnover of OsPIP2;1.Plant biotechnology journal · 2022Article
- From Soil Amendments to Controlling Autophagy: Supporting Plant Metabolism under Conditions of Water Shortage and Salinity.Plants (Basel, Switzerland) · 2022Review
- Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1).eLife · 2022Article
- Hormonal and environmental signaling pathways target membrane water transport.Plant physiology · 2021Review
16 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Arabidopsis thaliana multi-stress regulator TSPO is transiently induced by abiotic stresses. The final destination of this polytopic membrane protein is the Golgi apparatus, where its accumulation is strictly regulated, and TSPO is downregulated through a selective autophagic pathway. TSPO-related proteins regulate the physiology of the cell by generating functional protein complexes. A split-ubiquitin screen for potential TSPO interacting partners uncovered a plasma membrane aquaporin, PIP2;7. Pull-down assays and fluorescence imaging approaches revealed that TSPO physically interacts with PIP2;7 at the endoplasmic reticulum and Golgi membranes in planta. Intriguingly, constitutive expression of fluorescently tagged PIP2;7 in TSPO-overexpressing transgenic lines resulted in patchy distribution of the fluorescence, reminiscent of the pattern of constitutively expressed yellow fluorescent protein-TSPO in Arabidopsis. Mutational stabilization of TSPO or pharmacological inhibition of the autophagic pathway affected concomitantly the detected levels of PIP2;7, suggesting that the complex containing both proteins is degraded through the autophagic pathway. Coexpression of TSPO and PIP2;7 resulted in decreased levels of PIP2;7 in the plasma membrane and abolished the membrane water permeability mediated by transgenic PIP2;7. Taken together, these data support a physiological role for TSPO in regulating the cell-surface expression of PIP2;7 during abiotic stress conditions through protein-protein interaction and demonstrate an aquaporin regulatory mechanism involving TSPO.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.