ArticleAnnals of botany2015
Aphid-repellent pheromone E-β-farnesene is generated in transgenic Arabidopsis thaliana over-expressing farnesyl diphosphate synthase2.
Article in Annals of botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 38 citations in OpenAlex.
- Unravelling the molecular mechanisms of vegetative-to-reproductive transition in Cynara cardunculus by RNA-Seq analysis.Plant molecular biology · 2026Article
- Harnessing semiochemicals for parasitoid-based biological control: from laboratory identification to field applications.Crop health · 2025Review
- Molecular and genetic insights into secondary metabolic regulation underlying insect-pest resistance in legumes.Functional & integrative genomics · 2023Review
- Functional Characterization of a (International journal of molecular sciences · 2023Article
- Taxonomic Insights and Its Type Cyclization Correlation of Volatile Sesquiterpenes inMolecules (Basel, Switzerland) · 2021Review
- Biochemistry of Terpenes and Recent Advances in Plant Protection.International journal of molecular sciences · 2021Review
- Asymmetry in Herbivore Effector Responses: Caterpillar Frass Effectors Reduce Performance of a Subsequent Herbivore.Journal of chemical ecology · 2020Article
- Functional Gene Network of Prenyltransferases inMolecules (Basel, Switzerland) · 2019Review
- Transposition favors the generation of large effect mutations that may facilitate rapid adaption.Nature communications · 2019Article
- Article
- Harnessing enzyme plasticity for the synthesis of oxygenated sesquiterpenoids.Beilstein journal of organic chemistry · 2019Article
- Proteomic and metabolomic analyses provide insight into the off-flavour of fruits from citrus trees infected with 'Horticulture research · 2019Article
- Acibenzolar-Frontiers in plant science · 2018Article
- Suppressing Farnesyl Diphosphate Synthase Alters Chloroplast Development and Triggers Sterol-Dependent Induction of Jasmonate- and Fe-Related Responses.Plant physiology · 2016Article
- The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89).Biological research · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsPlant-synthesized sesquiterpenes play a pivotal role in chemotactic interactions with insects. Biosynthesis of functionally diverse sesquiterpenes is dependent on the availability of a pool of the precursor farnesyldiphosphate (FDP). In Arabidopsis thaliana, FPS2, encoding cytosolic farnesyldiphosphate synthase, is implicated in the synthesis of cytosolic FDP, but it is not known whether enhanced levels of FDP have a commensurate effect on sesquiterpene-mediated defence responses. This study examined transgenic arabidopsis plants generated to over-express FPS2 in order to determine if any effects could be observed in the response of aphids, Myzus persicae.
methodsTransgenic arabidopsis plants were generated to over-express FPS2 to produce FPS2 in either the cytosol or the chloroplasts. Morphochemical analyses of the transgenic plants were carried out to detremine growth responses of roots and shoots, and for GC-MS profiling of sesquiterpenes. Aphid response to hyrdo-distillate extracts and head-space volatiles from transgenic plants was assessed using a bioassay. KEY
resultsEither over-expression of FPS2 in the cytosol or targetting of its translated product to chlorplasts resulted in stimulatory growth responses of transgenic arabidopsis at early and late developmental stages. GC-MS analysis of hydro-distillate extracts from aerial parts of the plants revealed biosynthesis of several novel sesquiterpenes, including E-β-farnesene, an alarm pheromone of aphids. Both entrapped volatiles and hydro-distillate extracts of the transgenic leaves triggered agitation in aphids, which was related to both time and dose of exposure.
conclusionsOver-expression of FPS2 in the cytosol and targeting of its translated product to chloroplasts in arabidopsis led to synthesis of several novel sesquiterpenes, including E-β-farnesene, and induced alarm responses in M. persicae. The results suggest a potential for engineering aphid-resistant strains of arabidopsis.
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