ArticleJournal of synchrotron radiation2015
Functionalized synchrotron in-line phase-contrast computed tomography: a novel approach for simultaneous quantification of structural alterations and localization of barium-labelled alveolar macrophages within mouse lung samples.
Article in Journal of synchrotron radiation, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Quantitative 3D assessment of muscle dystrophy through X-ray phase-contrast tomography.Scientific reports · 2025Article
- 3DMPR - a robust morphological approach for applying phase retrieval in proximity to highly attenuating objects in computed tomography.Journal of synchrotron radiation · 2025Article
- Non-radioactive imaging strategies forPhysical sciences reviews · 2023Article
- X-ray-Based Techniques to Study the Nano-Bio Interface.ACS nano · 2021Article
- Diagnostic and Therapeutic Nanomedicine.Advances in experimental medicine and biology · 2021Article
- Three-dimensional assessment of bronchiectasis in a mouse model of mucociliary clearance disorder.ERJ open research · 2021Article
- Optimising complementary soft tissue synchrotron X-ray microtomography for reversibly-stained central nervous system samples.Scientific reports · 2018Article
- 3D imaging of cells in scaffolds: direct labelling for micro CT.Journal of materials science. Materials in medicine · 2018Article
- Nanotechnology for Cancer Therapy Based on Chemotherapy.Molecules (Basel, Switzerland) · 2018Review
- MÖNCH detector enables fast and low-dose free-propagation phase-contrast computed tomography of in situ mouse lungs.Journal of synchrotron radiation · 2018Article
- Therapeutic Fluorescent Hybrid Nanoparticles for Traceable Delivery of Glucocorticoids to Inflammatory Sites.Theranostics · 2018Article
- Diverse Applications of Nanomedicine.ACS nano · 2017Review
- X-Ray based Lung Function measurement-a sensitive technique to quantify lung function in allergic airway inflammation mouse models.Scientific reports · 2016Article
- Structural and transcriptomic response to antenatal corticosteroids in an Erk3-null mouse model of respiratory distress.American journal of obstetrics and gynecology · 2016Article
- Phase-contrast computed tomography for quantification of structural changes in lungs of asthma mouse models of different severity.Journal of synchrotron radiation · 2015Article
- Phase-contrast zoom tomography reveals precise locations of macrophages in mouse lungs.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 8 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functionalized computed tomography (CT) in combination with labelled cells is virtually non-existent due to the limited sensitivity of X-ray-absorption-based imaging, but would be highly desirable to realise cell tracking studies in entire organisms. In this study we applied in-line free propagation X-ray phase-contrast CT (XPCT) in an allergic asthma mouse model to assess structural changes as well as the biodistribution of barium-labelled macrophages in lung tissue. Alveolar macrophages that were barium-sulfate-loaded and fluorescent-labelled were instilled intratracheally into asthmatic and control mice. Mice were sacrificed after 24 h, lungs were kept in situ, inflated with air and scanned utilizing XPCT at the SYRMEP beamline (Elettra Synchrotron Light Source, Italy). Single-distance phase retrieval was used to generate data sets with ten times greater contrast-to-noise ratio than absorption-based CT (in our setup), thus allowing to depict and quantify structural hallmarks of asthmatic lungs such as reduced air volume, obstruction of airways and increased soft-tissue content. Furthermore, we found a higher concentration as well as a specific accumulation of the barium-labelled macrophages in asthmatic lung tissue. It is believe that XPCT will be beneficial in preclinical asthma research for both the assessment of therapeutic response as well as the analysis of the role of the recruitment of macrophages to inflammatory sites.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.