ArticleThe Journal of cell biology1989
Identification of actin nucleation activity and polymerization inhibitor in ameboid cells: their regulation by chemotactic stimulation.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 68 citations in OpenAlex.
- Caffeine inhibits PI3K and mTORC2 in Dictyostelium and differentially affects multiple other cAMP chemoattractant signaling effectors.Molecular and cellular biochemistry · 2019Article
- Protein kinase A regulates the Ras, Rap1 and TORC2 pathways in response to the chemoattractant cAMP inJournal of cell science · 2017Article
- Cofilin is a pH sensor for actin free barbed end formation: role of phosphoinositide binding.The Journal of cell biology · 2008Article
- Cdc42 is required for EGF-stimulated protrusion and motility in MTLn3 carcinoma cells.Journal of cell science · 2007Article
- Phospholipase C and cofilin are required for carcinoma cell directionality in response to EGF stimulation.The Journal of cell biology · 2004Article
- Role of Rac in controlling the actin cytoskeleton and chemotaxis in motile cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Assembly and function of the actin cytoskeleton of yeast: relationships between cables and patches.The Journal of cell biology · 1998Article
- Capping protein terminates but does not initiate chemoattractant-induced actin assembly in Dictyostelium.The Journal of cell biology · 1997Article
- Role of actin polymerization and adhesion to extracellular matrix in Rac- and Rho-induced cytoskeletal reorganization.The Journal of cell biology · 1997Article
- Bundling of actin filaments by elongation factor 1 alpha inhibits polymerization at filament ends.The Journal of cell biology · 1996Article
- Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides.The Journal of cell biology · 1996Article
- Coordinated regulation of platelet actin filament barbed ends by gelsolin and capping protein.The Journal of cell biology · 1996Article
- EGF stimulates lamellipod extension in metastatic mammary adenocarcinoma cells by an actin-dependent mechanism.Clinical & experimental metastasis · 1996Article
- The Dictyostelium cytoskeleton.Experientia · 1995Review
- Actin filament barbed-end capping activity in neutrophil lysates: the role of capping protein-beta 2.Molecular biology of the cell · 1995Article
- A talin homologue of Dictyostelium rapidly assembles at the leading edge of cells in response to chemoattractant.The Journal of cell biology · 1995Article
- Genetic deletion of ABP-120 alters the three-dimensional organization of actin filaments in Dictyostelium pseudopods.The Journal of cell biology · 1995Article
- Anti-tumor antibody BR96 blocks cell migration and binds to a lysosomal membrane glycoprotein on cell surface microspikes and ruffled membranes.The Journal of cell biology · 1994Article
- Role of actin polymerization in cell locomotion: molecules and models.American journal of respiratory cell and molecular biology · 1993Review
- Thymosin beta 4 sequesters the majority of G-actin in resting human polymorphonuclear leukocytes.The Journal of cell biology · 1992Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Actin polymerization occurs in amebae of Dictyostelium discoideum after chemotactic stimulation (Hall, A. L., A. Schlein, and J. Condeelis. 1988. J. Cell. Biochem. 37:285-299). When cells are lysed with Triton X-100 during stimulation, an actin nucleation activity is detected in lysates by measuring the rate of pyrene-labeled actin polymerization. This stimulated nucleation activity is closely correlated with actin polymerization observed in vivo in its kinetics, developmental regulation, and cytochalasin D sensitivity. Actin polymerization is coordinate with pseudopod extension in synchronized populations of cells and is correlated with the accumulation of F actin in pseudopods. The stimulated actin nucleation activity is present in low-speed pellets from Triton lysates (cytoskeletons) within 3 s of stimulation and is stable compared with the nucleation activity of whole cell lysates. Low-speed supernatants contain a reversible inhibitor of the actin nucleation activity that is itself regulated by chemotactic stimulation. Neither activity requires Ca2+ and both are fully expressed in 10 mM EGTA. Fractions containing the inhibitor do not sever actin filaments but do inhibit actin polymerization that is seeded by fragments of purified F actin. These results indicate that chemotactic stimulation of Dictyostelium discoideum generates both an actin-nucleating activity and an actin-polymerization inhibitor, and suggest that the parallel regulation of these two activities leads to the transient phases of actin polymerization observed in vivo. The different compartmentation of these two activities may account for polarized pseudopod extension in gradients of chemoattractant.
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