ArticlePflugers Archiv : European journal of physiology2015
Impedance analysis of GPCR-mediated changes in endothelial barrier function: overview and fundamental considerations for stable and reproducible measurements.
Article in Pflugers Archiv : European journal of physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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The trial behind it
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Who cites it
58 citing papers in PubMed.
- vascr: An R-based toolkit for rapid and robust analysis of cellular impedance sensing data.European biophysics journal : EBJ · 2026Article
- Noninvasive real-time monitoring of cellular spatiotemporal dynamics via machine learning-enhanced electrical impedance spectroscopy.Science advances · 2025Article
- In Vitro Modeling of Interorgan Crosstalk: Multi-Organ-on-a-Chip for Studying Cardiovascular-Kidney-Metabolic Syndrome.Circulation research · 2025Review
- Vascular Microphysiological System for Investigating Endothelial Barrier Function During Organ Preservation and Reperfusion.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Impedance mapping with high-density microelectrode array chips reveals dynamic heterogeneity of in vitro epithelial barriers.Scientific reports · 2025Article
- Article
- Rap1A Modulates Store-Operated Calcium Entry in the Lung Endothelium: A Novel Mechanism Controlling NFAT-Mediated Vascular Inflammation and Permeability.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- TRPV4 Mediates Alveolar Epithelial Barrier Integrity and Induces ADAM10-Driven E-Cadherin Shedding.Cells · 2024Article
- Cell-Electrode Models for Impedance Analysis of Epithelial and Endothelial Monolayers Cultured on Microelectrodes.Sensors (Basel, Switzerland) · 2024Article
- Modulation of the Blood-Brain Barrier by Sigma-1R Activation.International journal of molecular sciences · 2024Article
- Cortactin is in a complex with VE-cadherin and is required for endothelial adherens junction stability through Rap1/Rac1 activation.Scientific reports · 2024Article
- Investigation of the functional impact of CHED- and FECD4-associated SLC4A11 mutations in human corneal endothelial cells.PloS one · 2024Article
- NanoIEA: A Nanopatterned Interdigitated Electrode Array-Based Impedance Assay for Real-Time Measurement of Aligned Endothelial Cell Barrier Functions.Advanced healthcare materials · 2024Article
- Odorant Binding Causes Cytoskeletal Rearrangement, Leading to Detectable Changes in Endothelial and Epithelial Barrier Function and Micromotion.Biosensors · 2023Article
- Combining Electrostimulation with Impedance Sensing to Promote and Track Osteogenesis within a Titanium Implant.Biomedicines · 2023Article
- The Effects of Mucopolysaccharide Polysulfate on Steroid-Induced Tight Junction Barrier Dysfunction in Human Epidermal Keratinocytes and a 3D Skin Model.Skin pharmacology and physiology · 2023Article
- Functional optimization of electric cell-substrate impedance sensing (ECIS) using human corneal epithelial cells.Scientific reports · 2022Article
- Blocking of P2X7r Reduces Mitochondrial Stress Induced by Alcohol and Electronic Cigarette Exposure in Brain Microvascular Endothelial Cells.Antioxidants (Basel, Switzerland) · 2022Article
- Electric Cell-Substrate Impedance Sensing (ECIS) as a Platform for Evaluating Barrier-Function Susceptibility and Damage from Pulmonary Atelectrauma.Biosensors · 2022Article
- ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates.Biosensors · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The past 20 years has seen significant growth in using impedance-based assays to understand the molecular underpinning of endothelial and epithelial barrier function in response to physiological agonists and pharmacological and toxicological compounds. Most studies on barrier function use G protein-coupled receptor (GPCR) agonists which couple to fast and transient changes in barrier properties. The power of impedance-based techniques such as electric cell-substrate impedance sensing (ECIS) resides in its ability to detect minute changes in cell layer integrity label-free and in real-time ranging from seconds to days. We provide a comprehensive overview of the biophysical principles, applications, and recent developments in impedance-based methodologies. Despite extensive application of impedance analysis in endothelial barrier research, little attention has been paid to data analysis and critical experimental variables, which are both essential for signal stability and reproducibility. We describe the rationale behind common ECIS data presentation and interpretation and illustrate practical guidelines to improve signal intensity by adapting technical parameters such as electrode layout, monitoring frequency, or parameter (resistance versus impedance magnitude). Moreover, we discuss the impact of experimental parameters, including cell source, liquid handling, and agonist preparation on signal intensity and kinetics. Our discussions are supported by experimental data obtained from human microvascular endothelial cells challenged with three GPCR agonists, thrombin, histamine, and sphingosine-1-phosphate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.