ArticlePloS one2014
Characterisation of cyanobacterial bicarbonate transporters in E. coli shows that SbtA homologs are functional in this heterologous expression system.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 97 citations in OpenAlex.
- Uncovering a novel component in the cyanobacterial carbon concentration mechanism.Plant physiology · 2026Article
- Functional and structural insights into cyanobacterial COThe Plant journal : for cell and molecular biology · 2026Review
- Functional characterization of bicarbonate transporters from the cyanobacterial SbtA2 family and subsequent expression in tobacco.Journal of experimental botany · 2026Article
- The molecular mechanism of the cyanobacterial bicarbonate importer CmpABCD activated by the intracellular nitrate.Nature communications · 2026Article
- Heterologous expression in E. coli reveals that bicarbonate transporter BicA2 mediates carbon uptake in marine Prochlorococcus spp.The ISME journal · 2026Article
- Reprogramme the E. coli metabolism by engineering a functional carbon-fixation pathway.Journal of biological engineering · 2025Article
- Understanding carboxysomes to enhance carbon fixation in crops.Biochemical Society transactions · 2025Review
- Synthetic Genetic Elements Enable Rapid Characterization of Inorganic Carbon Uptake Systems inACS synthetic biology · 2025Article
- Molecular insights into the elevator-type mechanism of the cyanobacterial bicarbonate transporter BicA.Biophysical journal · 2025Article
- Engineering the cyanobacterial ATP-driven BCT1 bicarbonate transporter for functional targeting to C3 plant chloroplasts.Journal of experimental botany · 2024Article
- The Freshwater CyanobacteriumPlants (Basel, Switzerland) · 2024Article
- Adapting from Low to High: An Update to COPlants (Basel, Switzerland) · 2023Review
- Carbon signaling protein SbtB possesses atypical redox-regulated apyrase activity to facilitate regulation of bicarbonate transporter SbtA.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Roles of second messengers in the regulation of cyanobacterial physiology: the carbon-concentrating mechanism and beyond.microLife · 2023Review
- Trajectories for the evolution of bacterial COProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Diurnal metabolic control in cyanobacteria requires perception of second messenger signaling molecule c-di-AMP by the carbon control protein SbtB.Science advances · 2021Article
- Bacterial Adaptation by a Transposition Burst of an Invading IS Element.Genome biology and evolution · 2021Article
- Structures of cyanobacterial bicarbonate transporter SbtA and its complex with PII-like SbtB.Cell discovery · 2021Article
- Red/far-red light signals regulate the activity of the carbon-concentrating mechanism in cyanobacteria.Science advances · 2021Article
- Molecular mechanism underlying transport and allosteric inhibition of bicarbonate transporter SbtA.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacterial HCO3(-) transporters BCT1, SbtA and BicA are important components of cyanobacterial CO2-concentration mechanisms. They also show potential in applications aimed at improving photosynthetic rates and yield when expressed in the chloroplasts of C3 crop species. The present study investigated the feasibility of using Escherichia coli to assess function of a range of SbtA and BicA transporters in a heterologous expression system, ultimately for selection of transporters suitable for chloroplast expression. Here, we demonstrate that six β-forms of SbtA are active in E. coli, although other tested bicarbonate transporters were inactive. The sbtA clones were derived from Synechococcus sp. WH5701, Cyanobium sp. PCC7001, Cyanobium sp. PCC6307, Synechococcus elongatus PCC7942, Synechocystis sp. PCC6803, and Synechococcus sp. PCC7002. The six SbtA homologs varied in bicarbonate uptake kinetics and sodium requirements in E. coli. In particular, SbtA from PCC7001 showed the lowest uptake affinity and highest flux rate and was capable of increasing the internal inorganic carbon pool by more than 8 mM relative to controls lacking transporters. Importantly, we were able to show that the SbtB protein (encoded by a companion gene near sbtA) binds to SbtA and suppresses bicarbonate uptake function of SbtA in E. coli, suggesting a role in post-translational regulation of SbtA, possibly as an inhibitor in the dark. This study established E. coli as a heterologous expression and analysis system for HCO3(-) transporters from cyanobacteria, and identified several SbtA transporters as useful for expression in the chloroplast inner envelope membranes of higher plants.
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