ArticlePloS one2014
Lysostaphin-coated titan-implants preventing localized osteitis by Staphylococcus aureus in a mouse model.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.
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Who cites it
28 citing papers in PubMed, 2 syntheses or guidelines pooled it, 47 citations in OpenAlex.
- In Vivo Antibacterial Efficacy of Antimicrobial Peptides Modified Metallic Implants─Systematic Review and Meta-Analysis.ACS biomaterials science & engineering · 2022Pooled it
- Is coating of titanium implants effective at preventing Staphylococcus aureus infections? A meta-analysis of animal model studies.International orthopaedics · 2021Pooled it
- Eradication of MRSA biofilm-associated implant infections by low-immunogenic sustained-release lysostaphin fused to thermosensitive polypeptides.Nature communications · 2026Article
- Efficacy of lysostaphin-coated titanium plates on implant-associated MRSA osteitis in minipigs.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2024Article
- Implants coating strategies for antibacterial treatment in fracture and defect models: A systematic review of animal studies.Journal of orthopaedic translation · 2024Review
- Antibacterial and Anti-Biofilm Properties of Diopside Powder Loaded with Lysostaphin.Pathogens (Basel, Switzerland) · 2023Article
- Murine models of orthopedic infection featuringJournal of bone and joint infection · 2023Article
- Plate-associated localized osteitis in mini-pig by biofilm-forming Methicillin-resistant Staphylococcus aureus (MRSA): establishment of a novel experimental model.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2022Article
- Biomaterial-based delivery of antimicrobial therapies for the treatment of bacterial infections.Nature reviews. Materials · 2022Article
- An Enzybiotic Regimen for the Treatment of Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2021Article
- Review
- Staphylococcal Biofilms: Challenges and Novel Therapeutic Perspectives.Antibiotics (Basel, Switzerland) · 2021Review
- Evaluation of Topical Lysostaphin as a Novel Treatment for Instrumented Rhesus Macaques (Comparative medicine · 2020Article
- Therapeutics and delivery vehicles for local treatment of osteomyelitis.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Review
- A systematic review on current osteosynthesis-associated infection animal fracture models.Journal of orthopaedic translation · 2020Review
- A new sequential animal model for infection-related non-unions with segmental bone defect.BMC musculoskeletal disorders · 2020Article
- Production of Lysostaphin by Nonproprietary Method Utilizing a Promoter from Toxin-Antitoxin System.Molecular biotechnology · 2019Article
- Impact of Bacterial Infections on Osteogenesis: Evidence From In Vivo Studies.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Review
- Article
- Nonconventional Therapeutics againstMicrobiology spectrum · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increasing incidence of implant-associated infections induced by Staphylococcus aureus (SA) in combination with growing resistance to conventional antibiotics requires novel therapeutic strategies. In the current study we present the first application of the biofilm-penetrating antimicrobial peptide lysostaphin in the context of bone infections. In a standardized implant-associated bone infection model in mice beta-irradiated lysostaphin-coated titanium plates were compared with uncoated plates. Coating of the implant was established with a poly(D,L)-lactide matrix (PDLLA) comprising lysostaphin formulated in a stabilizing and protecting solution (SPS). All mice were osteotomized and infected with a defined count of SA. Fractures were fixed with lysostaphin-coated locking plates. Plates uncoated or PDLLA-coated served as controls. All mice underwent debridement and lavage on Days 7, 14, 28 to determine the bacterial load and local immune reaction. Fracture healing was quantified by conventional radiography. On Day 7 bacterial growth in the lavages of mice with lysostaphin-coated plates showed a significantly lower count to the control groups. Moreover, in the lysostaphin-coated plate groups complete fracture healing were observed on Day 28. The fracture consolidation was accompanied by a diminished local immune reaction. However, control groups developed an osteitis with lysis or destruction of the bone and an evident local immune response. The presented approach of terminally sterilized lysostaphin-coated implants appears to be a promising therapeutic approach for low grade infection or as prophylactic strategy in high risk fracture care e.g. after severe open fractures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.