ArticleProceedings of the National Academy of Sciences of the United States of America2015
Stochastic approach to the molecular counting problem in superresolution microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 118 citations in OpenAlex.
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- A Bayesian Model to Count the Number of Two-State Emitters in a Diffraction Limited Spot.Nano letters · 2025Article
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- Quantitative Single-Molecule Localization Microscopy.Annual review of biophysics · 2023Review
- Single-photon smFRET. I: Theory and conceptual basis.Biophysical reports · 2023Article
- Towards Unbiased Fluorophore Counting in Superresolution Fluorescence Microscopy.Nanomaterials (Basel, Switzerland) · 2023Article
- Single-molecule counting applied to the study of GPCR oligomerization.Biophysical journal · 2022Review
- Thermodynamic Driving Forces of Redox-Dependent CPR Insertion into Biomimetic Endoplasmic Reticulum Membranes.The journal of physical chemistry. B · 2022Article
- Diffraction-Limited Molecular Cluster Quantification with Bayesian Nonparametrics.Nature computational science · 2022Article
- Calibration-free counting of low molecular copy numbers in single DNA-PAINT localization clusters.Biophysical reports · 2021Article
- Quantifying molecular- to cellular-level forces in living cells.Journal of physics D: Applied physics · 2021Article
- Photobleaching step analysis for robust determination of protein complex stoichiometries.Molecular biology of the cell · 2021Article
- Estimating the dynamic range of quantitative single-molecule localization microscopy.Biophysical journal · 2021Article
- How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription.International journal of molecular sciences · 2021Review
- A pairwise distance distribution correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM.Nature methods · 2021Article
- PYMEVisualize: an open-source tool for exploring 3D super-resolution data.Nature methods · 2021Article
- Extraction of rapid kinetics from smFRET measurements using integrative detectors.Cell reports. Physical science · 2021Article
- Sample Preparation and Imaging Conditions Affect mEos3.2 Photophysics in Fission Yeast Cells.Biophysical journal · 2021Article
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4 authors at 3 institutions in 1 country.
Funding
Abstract
Superresolution imaging methods--now widely used to characterize biological structures below the diffraction limit--are poised to reveal in quantitative detail the stoichiometry of protein complexes in living cells. In practice, the photophysical properties of the fluorophores used as tags in superresolution methods have posed a severe theoretical challenge toward achieving this goal. Here we develop a stochastic approach to enumerate fluorophores in a diffraction-limited area measured by superresolution microscopy. The method is a generalization of aggregated Markov methods developed in the ion channel literature for studying gating dynamics. We show that the method accurately and precisely enumerates fluorophores in simulated data while simultaneously determining the kinetic rates that govern the stochastic photophysics of the fluorophores to improve the prediction's accuracy. This stochastic method overcomes several critical limitations of temporal thresholding methods.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.