ArticleJournal of bacteriology2015
Transcriptional regulation of the vanillate utilization genes (vanABK Operon) of Corynebacterium glutamicum by VanR, a PadR-like repressor.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 59 citations in OpenAlex.
- Precise Transcription Control Drives Optimized Catabolism of β-5-Type Lignin-Derived Dimers inJournal of agricultural and food chemistry · 2025Article
- EngineeredmSphere · 2025Article
- Catabolic System of Syringic Acid, a Key Intermediate of Lignin-Derived Aromatic Compounds, via a Novel Linear Pathway inJournal of agricultural and food chemistry · 2025Article
- The effects of PstR, a PadR family transcriptional regulatory factor, in Plesiomonas shigelloides are revealed by transcriptomics.BMC microbiology · 2024Article
- Identification of a PadR-type regulator essential for intracellular pathogenesis of Burkholderia pseudomallei.Scientific reports · 2021Article
- Re-engineering Plant Phenylpropanoid Metabolism With the Aid of Synthetic Biosensors.Frontiers in plant science · 2021Review
- A Timed Off-Switch for Dynamic Control of Gene Expression inFrontiers in bioengineering and biotechnology · 2021Article
- Physiological Response ofMicroorganisms · 2020Article
- Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress ofMicroorganisms · 2020Article
- The SyringateApplied and environmental microbiology · 2020Article
- Anaerobic Degradation of Syringic Acid by an Adapted Strain of Rhodopseudomonas palustris.Applied and environmental microbiology · 2020Article
- Regulation of vanillate and syringate catabolism by a MarR-type transcriptional regulator DesR in Sphingobium sp. SYK-6.Scientific reports · 2019Article
- Structural basis of effector and operator recognition by the phenolic acid-responsive transcriptional regulator PadR.Nucleic acids research · 2017Article
- NOT Gate Genetic Circuits to Control Gene Expression in Cyanobacteria.ACS synthetic biology · 2017Article
- Transcriptional Repressor PtvR Regulates Phenotypic Tolerance to Vancomycin in Streptococcus pneumoniae.Journal of bacteriology · 2017Article
- Gene Expression Analysis ofFrontiers in microbiology · 2017Article
- Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System.Applied and environmental microbiology · 2016Article
- The yjdF riboswitch candidate regulates gene expression by binding diverse azaaromatic compounds.RNA (New York, N.Y.) · 2016Article
- Global Transcriptomic Analysis of the Response of Corynebacterium glutamicum to Vanillin.PloS one · 2016Article
- Draft Genome Sequence of Streptomyces fradiae olg1-1, a Strain Resistant to Nitrone-Oligomycin.Genome announcements · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Corynebacterium glutamicum is able to utilize vanillate, the product of lignin degradation, as the sole carbon source. The vanillate utilization components are encoded by the vanABK operon. The vanA and vanB genes encode the subunits of vanillate O-demethylase, converting vanillate to protocatechuate, while VanK is the specific vanillate transporter. The vanABK operon is regulated by a PadR-type repressor, VanR. Heterologous gene expression and variations of the vanR open reading frame revealed that the functional VanR contains 192 residues (21 kDa) and forms a dimer, as analyzed by size exclusion chromatography. In vivo, ferulate, vanillin, and vanillate induced PvanABK in C. glutamicum, while only vanillate induced the activity of PvanABK in Escherichia coli lacking the ferulate catabolic system. Differential scanning fluorimetry verified that vanillate is the only effector of VanR. Interaction between the PvanABK DNA fragment and the VanR protein had an equilibrium dissociation constant (KD) of 15.1 ± 1.7 nM. The VanR-DNA complex had a dissociation rate constant (Kd) of (267 ± 23) × 10(-6) s(-1), with a half-life of 43.5 ± 3.6 min. DNase I footprinting localized the VanR binding site at PvanABK, extending from +9 to +45 on the coding strand. Deletion of the nucleotides +18 to +27 inside the VanR binding site rendered PvanABK constitutive. Fusion of the T7 promoter and the wild-type VanR operator, as well as its shortened versions, indicated that the inverted repeat AACTAACTAA(N4)TTAGGTATTT is the specific VanR binding site. It is proposed that the VanR-DNA complex contains two VanR dimers at the VanR operator.
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