ArticleJournal of bacteriology2015
Photoheterotrophic fluxome in Synechocystis sp. strain PCC 6803 and its implications for cyanobacterial bioenergetics.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 71 citations in OpenAlex.
- Investigating overflow metabolism in heterotrophic cultures of the green alga Chromochloris zofingiensis.Metabolic engineering · 2026Article
- Evaluating transcriptomic integration for cyanobacterial constraint-based metabolic modelling.Frontiers in bioinformatics · 2026Article
- Investigating Overflow Metabolism in Heterotrophic Cultures of the Green AlgabioRxiv : the preprint server for biology · 2025Article
- Improving productivity of citramalate from COBiotechnology for biofuels and bioproducts · 2024Article
- CRISPR interference screens reveal growth-robustness tradeoffs in Synechocystis sp. PCC 6803 across growth conditions.The Plant cell · 2023Article
- Citrate synthase from Cyanidioschyzon merolae exhibits high oxaloacetate and acetyl-CoA catalytic efficiency.Plant molecular biology · 2023Article
- Process Technologies of Cyanobacteria.Advances in biochemical engineering/biotechnology · 2023Article
- Tuning a high performing multiplexed-CRISPRiMetabolic engineering communications · 2022Article
- Quantitative insight into the metabolism of isoprene-producing Synechocystis sp. PCC 6803 using steady statePhotosynthesis research · 2022Article
- FirstFrontiers in plant science · 2022Article
- Patterning of the Autotrophic, Mixotrophic, and Heterotrophic Proteomes of Oxygen-Evolving CyanobacteriumFrontiers in microbiology · 2022Article
- Biochemical elucidation of citrate accumulation in Synechocystis sp. PCC 6803 via kinetic analysis of aconitase.Scientific reports · 2021Article
- Over-expression of an electron transport protein OmcS provides sufficient NADH for D-lactate production in cyanobacterium.Biotechnology for biofuels · 2021Article
- Investigation of carbon and energy metabolic mechanism of mixotrophy in Chromochloris zofingiensis.Biotechnology for biofuels · 2021Article
- Assessment of transcriptomic constraint-based methods for central carbon flux inference.PloS one · 2020Article
- Disruption of cyanobacterial γ-aminobutyric acid shunt pathway reduces metabolites levels in tricarboxylic acid cycle, but enhances pyruvate and poly(3-hydroxybutyrate) accumulation.Scientific reports · 2019Article
- Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase.Scientific reports · 2019Article
- A Hard Day's Night: Cyanobacteria in Diel Cycles.Trends in microbiology · 2019Review
- Extracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy.Scientific reports · 2019Article
- Biophotovoltaics: Green Power Generation From Sunlight and Water.Frontiers in microbiology · 2019Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study investigated metabolic responses in Synechocystis sp. strain PCC 6803 to photosynthetic impairment. We used 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU; a photosystem II inhibitor) to block O2 evolution and ATP/NADPH generation by linear electron flow. Based on (13)C-metabolic flux analysis ((13)C-MFA) and RNA sequencing, we have found that Synechocystis sp. PCC 6803 employs a unique photoheterotrophic metabolism. First, glucose catabolism forms a cyclic route that includes the oxidative pentose phosphate (OPP) pathway and the glucose-6-phosphate isomerase (PGI) reaction. Glucose-6-phosphate is extensively degraded by the OPP pathway for NADPH production and is replenished by the reversed PGI reaction. Second, the Calvin cycle is not fully functional, but RubisCO continues to fix CO2 and synthesize 3-phosphoglycerate. Third, the relative flux through the complete tricarboxylic acid (TCA) cycle and succinate dehydrogenase is small under heterotrophic conditions, indicating that the newly discovered cyanobacterial TCA cycle (via the γ-aminobutyric acid pathway or α-ketoglutarate decarboxylase/succinic semialdehyde dehydrogenase) plays a minimal role in energy metabolism. Fourth, NAD(P)H oxidation and the cyclic electron flow (CEF) around photosystem I are the two main ATP sources, and the CEF accounts for at least 40% of total ATP generation from photoheterotrophic metabolism (without considering maintenance loss). This study not only demonstrates a new topology for carbohydrate oxidation but also provides quantitative insights into metabolic bioenergetics in cyanobacteria.
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