Evidence map›Paper›PMID 25534037›Full record

ArticleLipids2015

BMP7 drives human adipogenic stem cells into metabolically active beige adipocytes.

Meshail Okla, Jung-Heun Ha, Ryan E Temel, Soonkyu Chung

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lipids, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 2 pooled it
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 2 syntheses or guidelines pooled it, 64 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Review
  5. Review
  6. Review
  7. Review
  8. AAV-mediated BMP7 gene therapy counteracts insulin resistance and obesity.Molecular therapy. Methods & clinical development · 2022
    Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. Review
  14. Article
  15. Signaling Pathways Regulating Thermogenesis.Frontiers in endocrinology · 2021
    Review
  16. Review
  17. Review
  18. Review
  19. Impact of adipokines and myokines on fat browning.Journal of physiology and biochemistry · 2020
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Meshail OklaDepartment of Nutrition and Health Sciences, University of Nebraska, 316G Ruth Leverton Hall, Lincoln, NE, 68583, USA.
Jung-Heun Ha
Ryan E Temel
Soonkyu Chung
University of Florida · USUniversity of Kentucky · USUniversity of Nebraska–Lincoln · US

Funding

The role of stress in the fetal origin of obesity and metabolic dysfunctionP20GM104320 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI ZEMPLENI, JANOS · 2014 to 2024
$24.9M
UF CLINICAL AND TRANSLATIONAL SCIENCE AWARDUL1RR029890 · NCRR · UNIVERSITY OF FLORIDA · PI NELSON, DAVID R · 2009 to 2011
$14.4M
Mechanisms For PPARdelta Agonist-Induced Elevation of HDL in Non-Human PrimatesR00HL088528 · NHLBI · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI TEMEL, RYAN EUGENE · 2009 to 2011
$743k
NCRR NIH HHS 1UL1RR029890NCRR NIH HHS UL1 RR029890NHLBI NIH HHS R00 HL088528NHLBI NIH HHS R00HL088528NIGMS NIH HHS NIH-1P20GM104320NIGMS NIH HHS P20 GM104320
6 · The paper itself

Abstract

Adult humans have a substantial amount of inducible-brown (or beige) fat, which is associated with increased energy expenditure and reduced weight gain via thermogenesis. Despite the identification of key regulators of beige adipogenesis, impacts of dietary factors on adaptive thermogenesis are largely unknown, partly due to a lack of validated human cell models. Bone morphogenetic protein 7 (BMP7) is known to promote brown adipogenesis in rodent and human progenitor cells. However, controversy still surrounds the cellular identity in BMP7-mediated transition of white to brown adipocytes. The aim of this study was to confirm BMP7-derived human adipocytes as a relevant in vitro model of human beige adipocyte by verifying the cellular lineage and metabolic activity. In this study, we hypothesized that pre-exposure of the stromal vascular (SV) fraction of primary human adipogenic precursor cells (hASC) to BMP7 would convert metabolically active brown adipocytes. Our results showed that exposure of hASC to human BMP7 was associated with significant escalation of (1) UCP1 gene expression, a signature gene of brown adipocytes, (2) beige specific marker gene expression (i.e., CD137 and TMEM26), (3) glucose and fatty acid uptake, and (4) basal and cAMP-stimulated oxygen consumption rate compared to white adipocyte control. Taken together, we demonstrated that BMP7 mediates conversion of hASC into metabolically active beige adipocytes. By confirming the cellular identity and metabolic activity, this BMP7-induced human beige adipocytes from hASC should aid in the discovery and assessment of bioactive molecules to promote adaptive thermogenesis.

Indexed as

AdipogenesisAdipocytes, BrownAdultAnimalsBone Morphogenetic Protein 7Cells, CulturedChlorocebus aethiopsFemaleGene Expression Regulation, DevelopmentalHumansIon ChannelsMaleMitochondrial ProteinsStem CellsUncoupling Protein 1BMP7 protein, humanBone Morphogenetic Protein 7Ion ChannelsMitochondrial ProteinsUCP1 protein, humanUncoupling Protein 1

Identifiers

PMID25534037
PMCPMC4306630
OpenAlexW2098926154

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.