ArticleNeurobiology of disease2015
Metabolic responses differentiate between interictal, ictal and persistent epileptiform activity in intact, immature hippocampus in vitro.
Article in Neurobiology of disease, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Intermittent fasting reduces interictal epileptiform discharges and hippocampal reactive astrogliosis during electrical kindling epileptogenesis.Metabolic brain disease · 2025Article
- Metabolic Adaptation in Epilepsy: From Acute Response to Chronic Impairment.International journal of molecular sciences · 2024Article
- Function and development of interneurons involved in brain tissue oxygen regulation.Frontiers in molecular neuroscience · 2022Review
- Brain tissue oxygen dynamics while mimicking the functional deficiency of interneurons.Frontiers in cellular neuroscience · 2022Article
- Contrasting Metabolic Insufficiency in Aging and Dementia.Aging and disease · 2021Review
- The circadian dynamics of the hippocampal transcriptome and proteome is altered in experimental temporal lobe epilepsy.Science advances · 2020Article
- Bioenergetic Mechanisms of Seizure Control.Frontiers in cellular neuroscience · 2018Review
- Dysregulated Glucose Metabolism as a Therapeutic Target to Reduce Post-traumatic Epilepsy.Frontiers in cellular neuroscience · 2018Review
- Effect of temperature on FAD and NADH-derived signals and neurometabolic coupling in the mouse auditory and motor cortex.Pflugers Archiv : European journal of physiology · 2017Article
- Review
- Event-Associated Oxygen Consumption Rate Increases ca. Five-Fold When Interictal Activity Transforms into Seizure-Like Events In Vitro.International journal of molecular sciences · 2017Article
- Acute Increases in Protein O-GlcNAcylation Dampen Epileptiform Activity in Hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Epileptiform abnormalities predict delayed cerebral ischemia in subarachnoid hemorrhage.Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology · 2017Article
- Fluorescence lifetime microscopy of NADH distinguishes alterations in cerebral metabolismBiomedical optics express · 2017Article
- Interneurons contribute to the hemodynamic/metabolic response to epileptiform discharges.Journal of neurophysiology · 2016Article
- Feed the Beast: The Power of Metabolism in Seizures.Epilepsy currentsArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Interictal spikes, ictal responses, and status epilepticus are characteristic of abnormal neuronal activity in epilepsy. Since these events may involve different energy requirements, we evaluated metabolic function (assessed by simultaneous NADH and FAD+ imaging and tissue O2 recordings) in the immature, intact mouse hippocampus (P5-P7, in vitro) during spontaneous interictal spikes and ictal-like events (ILEs), induced by increased neuronal network excitability with either low Mg2+ media or decreased inhibition with bicuculline. In low Mg2+ medium NADH fluorescence showed a small decrease both during the interictal build-up leading to an ictal event and before ILE occurrences, but a large positive response during and after ILEs (up to 10% net change). Tissue O2 recordings (pO2) showed an oxygen dip (indicating oxygen consumption) coincident with each ILE at P5 and P7, closely matching an NADH fluorescence increase, indicating a large surge in oxidative metabolism. The ILE O2 dip was significantly larger at P7 as compared to P5 suggesting a higher metabolic response at P7. After several ILEs at P7, continuous, low voltage activity (late recurrent discharges: LRDs) occurred. During LRDs, whilst the epileptiform activity was relatively small (low voltage synchronous activity) oxygen levels remained low and NADH fluorescence elevated, indicating persistent oxygen utilization and maintained high metabolic demand. In bicuculline, NADH fluorescence levels decreased prior to the onset of epileptiform activity, followed by a slow positive phase, which persisted during interictal responses. Metabolic responses can thus differentiate between interictal, ictal-like and persistent epileptiform activity resembling status epilepticus, and confirm that spreading depression did not occur. These results demonstrate clear translational value to the understanding of metabolic requirements during epileptic conditions.
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