ArticleToxins2014
Staphylococcal enterotoxin H induced apoptosis of bovine mammary epithelial cells in vitro.
Article in Toxins, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- Case Report of Toxic Shock-like Syndrome Associated with MixedVeterinary sciences · 2025Article
- Virulence of Bacteria Causing Mastitis in Dairy Cows: A Literature Review.Microorganisms · 2025Review
- Staphylococcal Enterotoxins: Description and Importance in Food.Pathogens (Basel, Switzerland) · 2024Review
- The Yeast and Hypha Phases ofAnimals : an open access journal from MDPI · 2023Article
- Staphylococcal Enterotoxins Promote Virulence in Bacterial Keratitis.Investigative ophthalmology & visual science · 2023Article
- Exploring the Role ofToxins · 2022Review
- Diversity and pathogenesis of Staphylococcus aureus from bovine mastitis: current understanding and future perspectives.BMC veterinary research · 2022Review
- A culture-, amplification-independent, and rapid method for identification of pathogens and antibiotic resistance profile in bovine mastitis milk.Frontiers in microbiology · 2022Article
- miR-142-5p regulates lipopolysaccharide-induced bovine epithelial cell proliferation and apoptosis via targeting BAG5.Experimental and therapeutic medicine · 2021Article
- Anti-Vaccines · 2021Article
- Virulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Selective Host Cell Death byFrontiers in immunology · 2020Review
- Impacts of the Staphylococcal Enterotoxin H on the Apoptosis and lncRNAs in PC3 and ACHN.Molecular genetics, microbiology and virology : Molekulyarnaya genetika, mikrobiologiya i virusologiya · 2020Article
- Article
- Molecular modelling and docking analysis of pleurocidin (an antimicrobial peptide) like peptides with enterotoxin H from Klebsilla pneumonia.Bioinformation · 2019Article
- Immunochemical assay with monoclonal antibodies for detection of staphylococcal enterotoxin H.Journal of food and drug analysis · 2018Article
- Article
- An Investigation of the Innate Immune Response in Bovine Mammary Epithelial Cells Challenged by Prototheca zopfii.Mycopathologia · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcal enterotoxins (SEs) are powerful superantigenic toxins produced by Staphylococcus aureus (S. aureus). They can cause food poisoning and toxic shock. However, their impact on bovine mammary epithelial cells (bMECs) is still unknown. In this study, the distribution of SE genes was evaluated in 116 S. aureus isolates from bovine mastitis, and the most prevalent genes were seh (36.2%), followed by sei (12.1%), seg (11.2%), ser (4.3%), sec (3.4%), sea (2.6%) and sed (1.7%). To better understand the effect of staphylococcal enterotoxin H (SEH) on bMECs, the seh gene was cloned and inserted into the prokaryotic expression vector, pET28a, and transformed into Escherichia coli BL21 (DE3). The recombinant staphylococcal enterotoxin H (rSEH) was expressed and purified as soluble protein. Bioactivity analysis showed that rSEH possessed the activity of stimulating lymphocytes proliferation. The XTT assay showed that 100 μg/mL of rSEH produced the cytotoxic effect on bMECs, and fluorescence microscopy and flow cytometry analysis revealed that a certain dose of rSEH is effective at inducing bMECs apoptosis in vitro. This indicates that SEs can directly lead to cellular apoptosis of bMECs in bovine mastitis associated with S. aureus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.