ArticleNeuron2015
Stoichiometry and phosphoisotypes of hippocampal AMPA-type glutamate receptor phosphorylation.
Article in Neuron, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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Who cites it
41 citing papers in PubMed.
- A single high dose of dexamethasone affects the phosphorylation state of glutamate AMPA receptors in the human limbic system.Translational psychiatry · 2016Trial
- SIK3 controls synaptic localization of NMDA receptors by phosphorylating GluN1.Nature neuroscience · 2026Article
- Targeting UBE3A and downstream estrogen receptor-β signaling to restore oligodendroglial homeostasis in Angelman syndrome.bioRxiv : the preprint server for biology · 2026Article
- The GluA1 cytoplasmic tail regulates intracellular AMPA receptor trafficking and synaptic transmission onto dentate gyrus GABAergic interneurons, gating response to novelty.Molecular psychiatry · 2026Article
- The VGCC auxiliary subunit α2δ1 is an extracellular GluA1 interactor and regulates LTP, spatial memory, and seizure susceptibility.bioRxiv : the preprint server for biology · 2024Article
- The Chemokine CCL2 Promotes Excitatory Synaptic Transmission in Hippocampal Neurons via GluA1 Subunit Trafficking.Neuroscience bulletin · 2024Article
- Molecular Mechanisms of AMPA Receptor Trafficking in the Nervous System.International journal of molecular sciences · 2023Review
- Engineering memory with an extrinsically disordered kinase.Science advances · 2023Article
- Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation.CNS neuroscience & therapeutics · 2023Article
- Synaptic memory and CaMKII.Physiological reviews · 2023Review
- Functional and phosphoproteomic analysis of β-adrenergic receptor signaling at excitatory synapses in the CA1 region of the ventral hippocampus.Scientific reports · 2023Article
- Unraveling the mysteries of dendritic spine dynamics: Five key principles shaping memory and cognition.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2023Article
- CaMKII: a central molecular organizer of synaptic plasticity, learning and memory.Nature reviews. Neuroscience · 2022Review
- CaMKII binds both substrates and activators at the active site.Cell reports · 2022Article
- The Shaping of AMPA Receptor Surface Distribution by Neuronal Activity.Frontiers in synaptic neuroscience · 2022Review
- AMPA receptor trafficking and LTP: Carboxy-termini, amino-termini and TARPs.Neuropharmacology · 2021Review
- Sustained postsynaptic kainate receptor activation downregulates AMPA receptor surface expression and induces hippocampal LTD.iScience · 2021Article
- Phosphorylation of the AMPA receptor subunit GluA1 regulates clathrin-mediated receptor internalization.Journal of cell science · 2021Article
- CaMKII activation persistently segregates postsynaptic proteins via liquid phase separation.Nature neuroscience · 2021Article
- Gene-environment interactions mediate stress susceptibility and resilience through the CaMKIIβ/TARPγ-8/AMPAR pathway.iScience · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
It has been proposed that the AMPAR phosphorylation regulates trafficking and channel activity, thereby playing an important role in synaptic plasticity. However, the actual stoichiometry of phosphorylation, information critical to understand the role of phosphorylation, is not known because of the lack of appropriate techniques for measurement. Here, using Phos-tag SDS-PAGE, we estimated the proportion of phosphorylated AMPAR subunit GluA1. The level of phosphorylated GluA1 at S831 and S845, two major sites implicated in AMPAR regulation, is almost negligible. Less than 1% of GluA1 is phosphorylated at S831 and less than 0.1% at S845. Considering the number of AMPAR at each synapse, the majority of synapses do not contain any phosphorylated AMPAR. Also, we did not see evidence of GluA1 dually phosphorylated at S831 and S845. Neuronal stimulation and learning increased phosphorylation, but the proportion was still low. Our results impel us to reconsider the mechanisms underlying synaptic plasticity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.