ArticleThe Journal of biological chemistry2015
A direct role for ATP1A1 in unconventional secretion of fibroblast growth factor 2.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 63 citations in OpenAlex.
- Review
- Glycolytic activation of β-cell NaMolecular metabolism · 2026Article
- Plasma membrane transbilayer asymmetry of PI(4,5)PNature communications · 2025Article
- Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space.eLife · 2024Article
- Conventional and Unconventional Protein Secretion in Yeast and Animal Cells.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Functional Analysis and Clinical Importance of ATP1A1 in Colon Cancer.Annals of surgical oncology · 2023Article
- Role of PI(4,5)P2 and Cholesterol in Unconventional Protein Secretion.Advances in experimental medicine and biology · 2023Article
- Cholesterol promotes clustering of PI(4,5)P2 driving unconventional secretion of FGF2.The Journal of cell biology · 2022Article
- New developments in the biology of fibroblast growth factors.WIREs mechanisms of disease · 2022Review
- Article
- Unconventional Secretion, Gate to Homeoprotein Intercellular Transfer.Frontiers in cell and developmental biology · 2022Review
- A Role for Liquid-Ordered Plasma Membrane Nanodomains Coordinating the Unconventional Secretory Pathway of Fibroblast Growth Factor 2?Frontiers in cell and developmental biology · 2022Review
- Identification of cis-acting determinants mediating the unconventional secretion of tau.Scientific reports · 2021Article
- The Na,K-ATPase acts upstream of phosphoinositide PI(4,5)PCommunications biology · 2020Article
- The Cell Biology of Tau Secretion.Frontiers in molecular neuroscience · 2020Review
- FGF2-FGFR1 signaling regulates release of Leukemia-Protective exosomes from bone marrow stromal cells.eLife · 2019Article
- Single event visualization of unconventional secretion of FGF2.The Journal of cell biology · 2019Article
- Tau Secretion: Good and Bad for Neurons.Frontiers in neuroscience · 2019Review
- Defining the molecular mechanisms of HIV-1 Tat secretion: PtdIns(4,5)PTraffic (Copenhagen, Denmark) · 2018Review
- Robust diagnosis of Ewing sarcoma by immunohistochemical detection of super-enhancer-driven EWSR1-ETS targets.Oncotarget · 2018Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies proposed a role for the Na/K-ATPase in unconventional secretion of fibroblast growth factor 2 (FGF2). This conclusion was based upon pharmacological inhibition of FGF2 secretion in the presence of ouabain. However, neither independent experimental evidence nor a potential mechanism was provided. Based upon an unbiased RNAi screen, we now report the identification of ATP1A1, the α1-chain of the Na/K-ATPase, as a factor required for efficient secretion of FGF2. As opposed to ATP1A1, down-regulation of the β1- and β3-chains (ATP1B1 and ATP1B3) of the Na/K-ATPase did not affect FGF2 secretion, suggesting that they are dispensable for this process. These findings indicate that it is not the membrane potential-generating function of the Na/K-ATPase complex but rather a so far unidentified role of potentially unassembled α1-chains that is critical for unconventional secretion of FGF2. Consistently, in the absence of β-chains, we found a direct interaction between the cytoplasmic domain of ATP1A1 and FGF2 with submicromolar affinity. Based upon these observations, we propose that ATP1A1 is a recruitment factor for FGF2 at the inner leaflet of plasma membranes that may control phosphatidylinositol 4,5-bisphosphate-dependent membrane translocation as part of the unconventional secretory pathway of FGF2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.