ArticleThe Journal of biological chemistry2015
Direct interaction of CaVβ with actin up-regulates L-type calcium currents in HL-1 cardiomyocytes.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Molecular Partners of Voltage-Gated Calcium Channel β and αThe Journal of membrane biology · 2026Review
- Mapping the interaction surface between CaNature communications · 2025Article
- T- and L-Type Calcium Channels Maintain Calcium Oscillations in the Murine Zona Glomerulosa.Hypertension (Dallas, Tex. : 1979) · 2024Article
- Growth-rate dependency of ribosome abundance and translation elongation rate in Corynebacterium glutamicum differs from that in Escherichia coli.Nature communications · 2023Article
- Membrane manipulation by free fatty acids improves microbial plant polyphenol synthesis.Nature communications · 2023Article
- RyR2 regulates store-operated Ca2+ entry, phospholipase C activity, and electrical excitability in the insulinoma cell line INS-1.PloS one · 2023Article
- Nitric Oxide Sensing by a Blue Fluorescent Protein.Antioxidants (Basel, Switzerland) · 2022Article
- Article
- The βFrontiers in cardiovascular medicine · 2021Article
- Nanoenviroments of the β-Subunit of L-Type Voltage-Gated Calcium Channels in Adult Cardiomyocytes.Frontiers in cell and developmental biology · 2021Article
- Autophagy-Related Proteins GABARAP and LC3B Label Structures of Similar Size but Different Shape in Super-Resolution Imaging.Molecules (Basel, Switzerland) · 2019Article
- A Tripartite Interaction Among the Calcium Channel αFrontiers in cellular neuroscience · 2019Article
- Rapid Turnover of the Cardiac L-Type CaiScience · 2018Article
- Clarin-1 gene transfer rescues auditory synaptopathy in model of Usher syndrome.The Journal of clinical investigation · 2018Article
- Localization and functional consequences of a direct interaction between TRIOBP-1 and hERG proteins in the heart.Journal of cell science · 2018Article
- Altered profile of mRNA expression in atrioventricular node of streptozotocin‑induced diabetic rats.Molecular medicine reports · 2017Article
- BDNF/trkB Induction of Calcium Transients through CaFrontiers in molecular neuroscience · 2017Article
- Ca(2+)/calmodulin-activated phosphodiesterase 1A is highly expressed in rabbit cardiac sinoatrial nodal cells and regulates pacemaker function.Journal of molecular and cellular cardiology · 2016Article
- Actin Filaments Regulate Exocytosis at the Hair Cell Ribbon Synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy.Scientific reports · 2015Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of the β-subunit (CaVβ) is required for normal function of cardiac L-type calcium channels, and its up-regulation is associated with heart failure. CaVβ binds to the α1 pore-forming subunit of L-type channels and augments calcium current density by facilitating channel opening and increasing the number of channels in the plasma membrane, by a poorly understood mechanism. Actin, a key component of the intracellular trafficking machinery, interacts with Src homology 3 domains in different proteins. Although CaVβ encompasses a highly conserved Src homology 3 domain, association with actin has not yet been explored. Here, using co-sedimentation assays and FRET experiments, we uncover a direct interaction between CaVβ and actin filaments. Consistently, single-molecule localization analysis reveals streaklike structures composed by CaVβ2 that distribute over several micrometers along actin filaments in HL-1 cardiomyocytes. Overexpression of CaVβ2-N3 in HL-1 cells induces an increase in L-type current without altering voltage-dependent activation, thus reflecting an increased number of channels in the plasma membrane. CaVβ mediated L-type up-regulation, and CaVβ-actin association is prevented by disruption of the actin cytoskeleton with cytochalasin D. Our study reveals for the first time an interacting partner of CaVβ that is directly involved in vesicular trafficking. We propose a model in which CaVβ promotes anterograde trafficking of the L-type channels by anchoring them to actin filaments in their itinerary to the plasma membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.