ArticleScientific reports2014
Asymmetric parental genome engineering by Cas9 during mouse meiotic exit.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed.
- Highly efficient and low-mosaicismiScience · 2026Article
- Double Electroporation Combined with Zona Pellucida Removal Improves Biallelic Genome Editing Efficiency in Porcine Embryos.Animals : an open access journal from MDPI · 2026Article
- Review
- Effects of the timing of electroporation duringVeterinary and animal science · 2022Article
- Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection.Veterinary world · 2022Article
- Cytoplasmic Injection of Zygotes to Genome Edit Naturally Occurring Sequence Variants Into Bovine Embryos.Frontiers in genetics · 2022Article
- Genomic imprinting in mouse blastocysts is predominantly associated with H3K27me3.Nature communications · 2021Article
- The dawn of non-human primate models for neurodevelopmental disorders.Current opinion in genetics & development · 2020Review
- Tracking intracellular forces and mechanical property changes in mouse one-cell embryo development.Nature materials · 2020Article
- Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence.Scientific reports · 2020Article
- Review
- Sepsis in the era of data-driven medicine: personalizing risks, diagnoses, treatments and prognoses.Briefings in bioinformatics · 2020Review
- Human germline genome editing.Nature cell biology · 2019Review
- Gene-edited babies: What went wrong and what could go wrong.PLoS biology · 2019Article
- Strategies for Efficient Genome Editing Using CRISPR-Cas9.Genetics · 2019Article
- Inter-homologue repair in fertilized human eggs?Nature · 2018Article
- Switchable genome editing via genetic code expansion.Scientific reports · 2018Article
- Gene Editing in Humans: Towards a Global and Inclusive Debate for Responsible Research.The Yale journal of biology and medicine · 2017Article
- Article
- Precision editing in the human embryo.Nature biotechnology · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
Mammalian genomes can be edited by injecting pronuclear embryos with Cas9 cRNA and guide RNA (gRNA) but it is unknown whether editing can also occur during the onset of embryonic development, prior to pronuclear embryogenesis. We here report Cas9-mediated editing during sperm-induced meiotic exit and the initiation of development. Injection of unfertilized, mouse metaphase II (mII) oocytes with Cas9 cRNA, gRNA and sperm enabled efficient editing of transgenic and native alleles. Pre-loading oocytes with Cas9 increased sensitivity to gRNA ~100-fold. Paternal allelic editing occurred as an early event: single embryo genome analysis revealed editing within 3 h of sperm injection, coinciding with sperm chromatin decondensation during the gamete-to-embryo transition but prior to pronucleus formation. Maternal alleles underwent editing after the first round of DNA replication, resulting in mosaicism. Asymmetric editing of maternal and paternal alleles suggests a novel strategy for discriminatory targeting of parental genomes.
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Registered trials
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