ArticlePloS one2014
Identification and molecular characterization of microneme 5 of Eimeria acervulina.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- What Do We Know about Surface Proteins of Chicken ParasitesLife (Basel, Switzerland) · 2023Review
- The microneme adhesive repeat domain of MIC3 protein determined the site specificity ofFrontiers in immunology · 2023Article
- Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities.Parasitology research · 2022Review
- Eimeria proteins: order amidst disorder.Parasites & vectors · 2022Review
- Actin depolymerizing factor-based nanomaterials: A novel strategy to enhanceFrontiers in immunology · 2022Article
- PLGA Nanospheres as Delivery Platforms forFrontiers in immunology · 2022Article
- Anticoccidial efficacy ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Trichinella spiralis: Knockdown of gamma interferon inducible lysosomal thiol reductase (GILT) results in the reduction of worm burden.PLoS neglected tropical diseases · 2021Article
- Establishment of RecombinantVaccines · 2021Article
- Evaluation of immunoprotective effects of recombinant protein and DNA vaccine based on Eimeria tenella surface antigen 16 and 22 in vivo.Parasitology research · 2021Article
- Haemonchus contortus: siRNA mediated knockdown of matrix metalloproteinase 12A (MMP-12) results in reduction of infectivity.Parasites & vectors · 2020Article
- Immunoproteomic and mass spectrometric analysis of Eimeria acervulina antigens recognized by antisera from chickens infected with E. acervulina, E. tenella or E. necatrix.Parasites & vectors · 2020Article
- Galectin Domain Containing Protein fromBiomolecules · 2020Article
- Molecular characterization of a potential receptor of Eimeria acervulina microneme protein 3 from chicken duodenal epithelial cells.Parasite (Paris, France) · 2020Article
- Succinate Coenzyme A Ligase Beta-Like Protein fromVaccines · 2019Article
- Toxoplasma gondii Histone 4 Affects Some Functions of Murine Ana-1 Macrophages In Vitro.The Journal of eukaryotic microbiology · 2018Article
- Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis.Oncotarget · 2017Article
- Characterization of a novel aspartyl protease inhibitor from Haemonchus contortus.Parasites & vectors · 2017Article
- Effects of RecombinantFrontiers in microbiology · 2017Article
- Molecular Characterization and Immune Protection of a New Conserved Hypothetical Protein of Eimeria tenella.PloS one · 2016Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, the microneme 5 gene of Eimeria acervulina (E. acervulina) (EaMIC5) was cloned and characterized. Specific primers for the rapid amplification of cDNA ends (RACE) were designed based on the expressed sequence tag (EST, GenBank Accession No. EH386430.1) to amplify the 3'- and 5'-ends of EaMIC5. The full length cDNA of this gene was obtained by overlapping the sequences of 3'- and 5'-extremities and amplification by reverse transcription PCR. Sequence analysis revealed that the open reading frame (ORF) of EaMIC5 was 336 bp and encoded a protein of 111 amino acids with 12.18 kDa. The ORF was inserted into pET-32a (+) to produce recombinant EaMIC5. Using western blotting assay, the recombinant protein was successfully recognized by the sera of chicks experimentally infected with E. acervulina, while the native protein in the somatic extract of sporozoites was as well detected by sera from rats immunized with the recombinant protein of EaMIC5. Immunofluorescence analysis using antibody against recombinant protein EaMIC5 indicated that this protein was expressed in the sporozoites and merozoites stages of E. acervulina. Animal challenge experiments demonstrated that the recombinant protein of EaMIC5 could significantly increase the average body weight gains, decrease the mean lesion scores and the oocyst outputs of the immunized chickens, and presented anti-coccidial index (ACI) more than 160. All the above results suggested that the EaMIC5 was a novel E. acervulina antigen and could be an effective candidate for the development of a new vaccine against this parasite.
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