ArticlePloS one2014
High-resolution melting of 12S rRNA and cytochrome b DNA sequences for discrimination of species within distinct European animal families.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 18 citations in OpenAlex.
- DNA Barcoding in Meat Authentication: Principles, Applications, and Future Perspectives.Foods (Basel, Switzerland) · 2025Review
- Feasibility of melting fingerprint obtained from ISSR-HRM curves for marine mammal species identification.PeerJ · 2021Article
- Detection of a G>C single nucleotide polymorphism within a repetitive DNA sequence by high-resolution DNA melting.International journal of legal medicine · 2016Article
- Article
- Mammalian species identification using ISSR-HRM technique.Science progressArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cheap and easy identification of species is necessary within multiple fields of molecular biology. The use of high-resolution melting (HRM) of DNA provides a fast closed-tube method for analysis of the sequence composition of the mitochondrial genes 12S rRNA and cytochrome b. We investigated the potential use of HRM for species identification within eleven different animal groups commonly found in Europe by animal-group-specific DNA amplification followed by DNA melting. Influence factors as DNA amount, additional single base alterations, and the existence of mixed samples were taken into consideration. Visual inspection combined with mathematical evaluation of the curve shapes did resolve nearly all species within an animal group. The assay can therefore not only be used for identification of animal groups and mixture analysis but also for species identification within the respective groups. The use of a universal 12S rRNA system additionally revealed a possible approach for species discrimination, mostly by exclusion. The use of the HRM assay showed to be a reliable, fast, and cheap method for species discrimination within a broad range of different animal species and can be used in a flexible "modular" manner depending on the question to be solved.
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