ArticlePloS one2014
Carbon-ion beam irradiation kills X-ray-resistant p53-null cancer cells by inducing mitotic catastrophe.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 44 citations in OpenAlex.
- Esophageal Cancer Cells Exhibit Heterogeneity in DNA Double-Strand Break Repair and G2/M Checkpoint Arrest Associated With Cell Viability After Ionizing Radiation.Advances in radiation oncology · 2026Article
- Carbon ion radiotherapy for mesonephric adenocarcinoma of the uterine cervix: a case report.Journal of medical case reports · 2024Article
- Analysis of the relationship between LET, γH2AX foci volume and cell killing effect of carbon ions using high-resolution imaging technology.Journal of radiation research · 2023Article
- Basic and translational research on carbon-ion radiobiology.American journal of cancer research · 2023Review
- Article
- Comparable radiation sensitivity in p53 wild-type and p53 deficient tumor cells associated with different cell death modalities.Cell death discovery · 2021Article
- Induction of Micronuclei in Cervical Cancer Treated with Radiotherapy.Journal of personalized medicine · 2020Article
- Relative Biological Effectiveness of Carbon Ions for Head-and-Neck Squamous Cell Carcinomas According to Human Papillomavirus Status.Journal of personalized medicine · 2020Article
- Heterogeneity of chondrosarcomas response to irradiations with X-rays and carbon ions: A comparative study on five cell lines.Journal of bone oncology · 2020Article
- Preliminary study on radiosensitivity to carbon ions in human breast cancer.Journal of radiation research · 2020Article
- p53 deficiency augments nucleolar instability after ionizing irradiation.Oncology reports · 2019Article
- Clustered DNA double-strand break formation and the repair pathway following heavy-ion irradiation.Journal of radiation research · 2019Review
- High linear energy transfer carbon-ion irradiation increases the release of the immune mediator high mobility group box 1 from human cancer cells.Journal of radiation research · 2018Article
- Mutational analysis of uterine cervical cancer that survived multiple rounds of radiotherapy.Oncotarget · 2018Article
- Etoposide radiosensitizes p53-defective cholangiocarcinoma cell lines independent of their GOncology letters · 2018Article
- Comparing Photon and Charged Particle Therapy Using DNA Damage Biomarkers.International journal of particle therapy · 2018Review
- Does Neutron Radiation Therapy Potentiate an Immune Response to Merkel Cell Carcinoma?International journal of particle therapy · 2018Article
- One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy.Journal of visualized experiments : JoVE · 2017Article
- Deciphering the Acute Cellular Phosphoproteome Response to Irradiation with X-rays, Protons and Carbon Ions.Molecular & cellular proteomics : MCP · 2017Article
- Inhibiting autophagy with chloroquine enhances the anti-tumor effect of high-LET carbon ions via ER stress-related apoptosis.Medical oncology (Northwood, London, England) · 2017Article
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and purposeTo understand the mechanisms involved in the strong killing effect of carbon-ion beam irradiation on cancer cells with TP53 tumor suppressor gene deficiencies. MATERIALS AND
methodsDNA damage responses after carbon-ion beam or X-ray irradiation in isogenic HCT116 colorectal cancer cell lines with and without TP53 (p53+/+ and p53-/-, respectively) were analyzed as follows: cell survival by clonogenic assay, cell death modes by morphologic observation of DAPI-stained nuclei, DNA double-strand breaks (DSBs) by immunostaining of phosphorylated H2AX (γH2AX), and cell cycle by flow cytometry and immunostaining of Ser10-phosphorylated histone H3.
resultsThe p53-/- cells were more resistant than the p53+/+ cells to X-ray irradiation, while the sensitivities of the p53+/+ and p53-/- cells to carbon-ion beam irradiation were comparable. X-ray and carbon-ion beam irradiations predominantly induced apoptosis of the p53+/+ cells but not the p53-/- cells. In the p53-/- cells, carbon-ion beam irradiation, but not X-ray irradiation, markedly induced mitotic catastrophe that was associated with premature mitotic entry with harboring long-retained DSBs at 24 h post-irradiation.
conclusionsEfficient induction of mitotic catastrophe in apoptosis-resistant p53-deficient cells implies a strong cancer cell-killing effect of carbon-ion beam irradiation that is independent of the p53 status, suggesting its biological advantage over X-ray treatment.
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