ArticlemAbs2015
Characterization of monoclonal antibody's binding kinetics using oblique-incidence reflectivity difference approach.
Article in mAbs, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Monitoring Endocytosis of Integral Membrane Proteins Using Western Blot-Based Detection of Biotinylated Antibody Uptake.Bio-protocol · 2025Article
- Sheet Protector Strategy for Western Blot to Reduce Antibody Consumption and Incubation Time.Biological procedures online · 2025Article
- Ultrasensitive Bead-Based Immunoassay for Real-Time Continuous Sample Flow Analysis.Biosensors · 2025Article
- A single-domain antibody library based on a stability-engineered human VH3 scaffold.Scientific reports · 2024Article
- Production of Antibodies to Peptide Targets Using Hybridoma Technology.Methods in molecular biology (Clifton, N.J.) · 2024Article
- T-cell engagers: model interrogation as a tool to quantify the interplay of relative affinity and target expression on trimer formation.Frontiers in pharmacology · 2024Article
- Two Decades of Arrayed Imaging Reflectometry for Sensitive, High-Throughput Biosensing.Biosensors · 2023Review
- Epitope Binning of Novel Monoclonal Anti F1 and Anti LcrV Antibodies and Their Application in a Simple, Short, HTRF Test for Clinical Plague Detection.Pathogens (Basel, Switzerland) · 2021Article
- Modeling the activation of the alternative complement pathway and its effects on hemolysis in health and disease.PLoS computational biology · 2020Article
- Probe-target hybridization depends on spatial uniformity of initial concentration condition across large-format chips.Scientific reports · 2020Article
- Development of a Molecular Imprinting-Based Surface Plasmon Resonance Biosensor for Rapid and Sensitive Detection ofFrontiers in cellular and infection microbiology · 2020Article
- Direct detection of bacteremia by exploiting host-pathogen interactions of lipoteichoic acid and lipopolysaccharide.Scientific reports · 2019Article
- Measuring Ligand Binding Kinetics to Membrane Proteins Using Virion Nano-oscillators.Journal of the American Chemical Society · 2018Article
- Real-time detection of dielectric anisotropy or isotropy in unconventional oil-gas reservoir rocks supported by the oblique-incidence reflectivity difference technique.Scientific reports · 2016Article
- Transcription factors as readers and effectors of DNA methylation.Nature reviews. Genetics · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Monoclonal antibodies (mAbs) against human proteins are the primary protein capture reagents for basic research, diagnosis, and molecular therapeutics. The 2 most important attributes of mAbs used in all of these applications are their specificity and avidity. While specificity of a mAb raised against a human protein can be readily defined based on its binding profile on a human proteome microarray, it has been a challenge to determine avidity values for mAbs in a high-throughput and cost-effective fashion. To undertake this challenge, we employed the oblique-incidence reflectivity difference (OIRD) platform to characterize mAbs in a protein microarray format. We first systematically determined the Kon and Koff values of 50 mAbs measured with the OIRD method and deduced the avidity values. Second, we established a multiplexed approach that simultaneously measured avidity values of a mixture of 9 mono-specific mAbs that do not cross-react to the antigens. Third, we demonstrated that avidity values of a group of mAbs could be sequentially determined using a flow-cell device. Finally, we implemented a sequential competition assay that allowed us to bin multiple mAbs that recognize the same antigens. Our study demonstrated that OIRD offers a high-throughput and cost-effective platform for characterization of the binding kinetics of mAbs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.