ArticleJournal of structural biology2015
Seeing tobacco mosaic virus through direct electron detectors.
Article in Journal of structural biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Using atomic force microscopy for physical virology: touching and manipulating single virus particles.Biophysical reviews · 2026Review
- DiameTR: A cryo-EM tool for diameter sorting of tubular samples.Journal of structural biology: X · 2025Article
- Getting Hold of the Tobamovirus Particle-Why and How? Purification Routes over Time and a New Customizable Approach.Viruses · 2024Review
- VitroJet: new features and case studies.Acta crystallographica. Section D, Structural biology · 2024Article
- Dynamical scattering in ice-embedded proteins in conventional and scanning transmission electron microscopy.IUCrJ · 2023Article
- Structural basis for gating mechanism of the human sodium-potassium pump.Nature communications · 2022Article
- Single-particle cryo-EM structures from iDPC-STEM at near-atomic resolution.Nature methods · 2022Article
- Ab initio reconstruction from one-dimensional crystal diffraction data.Acta crystallographica. Section A, Foundations and advances · 2022Article
- Article
- Cryo-electron tomography related radiation-damage parameters for individual-molecule 3D structure determination.Frontiers in chemistry · 2022Review
- Review
- A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants.Viruses · 2021Article
- Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- High-resolution cryo-EM structure of urease from the pathogen Yersinia enterocolitica.Nature communications · 2020Article
- Two particle-picking procedures for filamentous proteins: SPHIRE-crYOLO filament mode and SPHIRE-STRIPER.Acta crystallographica. Section D, Structural biology · 2020Article
- Phage liquid crystalline droplets form occlusive sheaths that encapsulate and protect infectious rod-shaped bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Elucidation of the viral disassembly switch of tobacco mosaic virus.EMBO reports · 2019Article
- FunctionalizedCancers · 2019Article
- CM01: a facility for cryo-electron microscopy at the European Synchrotron.Acta crystallographica. Section D, Structural biology · 2019Article
- Article
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Authors and funding
5 authors.
Funding
Abstract
With the introduction of direct electron detectors (DED) to the field of electron cryo-microscopy, a wave of atomic-resolution structures has become available. As the new detectors still require comparative characterization, we have used tobacco mosaic virus (TMV) as a test specimen to study the quality of 3D image reconstructions from data recorded on the two direct electron detector cameras, K2 Summit and Falcon II. Using DED movie frames, we explored related image-processing aspects and compared the performance of micrograph-based and segment-based motion correction approaches. In addition, we investigated the effect of dose deposition on the atomic-resolution structure of TMV and show that radiation damage affects negative carboxyl chains first in a side-chain specific manner. Finally, using 450,000 asymmetric units and limiting the effects of radiation damage, we determined a high-resolution cryo-EM map at 3.35Å resolution. Here, we provide a comparative case study of highly ordered TMV recorded on different direct electron detectors to establish recording and processing conditions that enable structure determination up to 3.2Å in resolution using cryo-EM.
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