ArticleMetabolism: clinical and experimental2015
Low levels of lipopolysaccharide modulate mitochondrial oxygen consumption in skeletal muscle.
Article in Metabolism: clinical and experimental, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 51 citations in OpenAlex.
- Trial
- Immune-Induced Fever and Psychogenic Hyperthermia Can Occur Independently of Uncoupling Protein 1-Mediated Brown Adipose Tissue Thermogenesis.Acta physiologica (Oxford, England) · 2026Article
- Article
- Tissue-resident bacteria in metabolic diseases: emerging evidence and challenges.Nature metabolism · 2024Review
- Sulforaphane Ameliorates High-Fat-Diet-Induced Metabolic Abnormalities in Young and Middle-Aged Obese Male Mice.Foods (Basel, Switzerland) · 2024Article
- High-intensity interval training attenuates impairment in regulatory protein machinery of mitochondrial quality control in skeletal muscle of diet-induced obese mice.Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 2024Article
- Protective Effects of the Chalcone-Based Derivative AN07 on Inflammation-Associated Myotube Atrophy Induced by Lipopolysaccharide.International journal of molecular sciences · 2022Article
- Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-γ.Inflammation · 2022Article
- Pharmacological inhibition of dynamin-related protein 1 attenuates skeletal muscle insulin resistance in obesity.Physiological reports · 2021Article
- Protective Effect of Mitochondria-Targeted Antioxidants against Inflammatory Response to Lipopolysaccharide Challenge: A Review.Pharmaceutics · 2021Review
- Impaired Intestinal Barrier and Tissue Bacteria: Pathomechanisms for Metabolic Diseases.Frontiers in endocrinology · 2021Review
- Overfeeding and Substrate Availability, But Not Age or BMI, Alter Human Satellite Cell Function.Nutrients · 2020Article
- Sustained maternal inflammation during the early third-trimester yields intrauterine growth restriction, impaired skeletal muscle glucose metabolism, and diminished β-cell function in fetal sheep1,2.Journal of animal science · 2019Article
- Angiotensin II suppresses autophagy and disrupts ultrastructural morphology and function of mitochondria in mouse skeletal muscle.Journal of applied physiology (Bethesda, Md. : 1985) · 2019Article
- β-Hydroxybutyrate protects from alcohol-induced liver injury via a Hcar2-cAMP dependent pathway.Journal of hepatology · 2018Article
- Deficiency of Soluble Epoxide Hydrolase Protects Cardiac Function Impaired by LPS-Induced Acute Inflammation.Frontiers in pharmacology · 2018Article
- Mitochondrial Function, Obesity, and Personalized Lifestyle Medicine.Integrative medicine (Encinitas, Calif.) · 2017Article
- Glutamate alleviates muscle protein loss by modulating TLR4, NODs, Akt/FOXO and mTOR signaling pathways in LPS-challenged piglets.PloS one · 2017Article
- Sepsis-Induced Cardiomyopathy: Oxidative Implications in the Initiation and Resolution of the Damage.Oxidative medicine and cellular longevity · 2017Review
- The complementary and divergent roles of uncoupling proteins 1 and 3 in thermoregulation.The Journal of physiology · 2016Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveWe have previously demonstrated that activation of toll-like receptor 4 (TLR4) in skeletal muscle results in an increased reliance on glucose as an energy source and a concomitant decrease in fatty acid oxidation under basal conditions. Herein, we examined the effects of lipopolysaccharide (LPS), the primary ligand for TLR4, on mitochondrial oxygen consumption in skeletal muscle cell culture and mitochondria isolated from rodent skeletal muscle. MATERIALS/
methodsSkeletal muscle cell cultures were exposed to LPS and oxygen consumption was assessed using a Seahorse Bioscience extracellular flux analyzer. Mice were also exposed to LPS and oxygen consumption was assessed in mitochondria isolated from skeletal muscle.
resultsAcute LPS exposure resulted in significant reductions in Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP)-stimulated maximal respiration (state 3u) and increased oligomycin induced state 4 (state 4O) respiration in C2C12 and human primary myotubes. These findings were observed in conjunction with increased mRNA of uncoupling protein 3 (UCP3), superoxide dismutase 2 (SOD2), and pyruvate dehydrogenase activity. The LPS-mediated changes in substrate oxidation and maximal mitochondrial respiration were prevented in the presence of the antioxidants N-acetylcysteine and catalase, suggesting a potential role of reactive oxygen species in mediating these effects. Mitochondria isolated from red gastrocnemius and quadriceps femoris muscle from mice injected with LPS also demonstrated reduced respiratory control ratio (RCR), and ADP- and FCCP-stimulated respiration.
conclusionLPS exposure in skeletal muscle alters mitochondrial oxygen consumption and substrate preference, which is absent when antioxidants are present.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.