ArticleCytotechnology2016
X/XO or H2O2 induced IPEC-J2 cell as a new in vitro model for studying apoptosis in post-weaning piglets.
Article in Cytotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Integrated Transcriptome and Metabolome Analysis of the Porcine Small Intestine During Weaning.Genes · 2025Article
- Butorphanol inhibits ferroptosis to attenuate PC12 cell injury by blocking JNK/p38 signaling.Experimental and therapeutic medicine · 2024Article
- Lipopolysaccharide promotes apoptosis and oxidative injury of porcine small intestinal epithelial cells by down-regulating the expression of glutamine transporter ASCT2.Journal of animal science · 2023Article
- Alfalfa saponins inhibit oxidative stress-induced cell apoptosis through the MAPK signaling pathway.Redox report : communications in free radical research · 2022Article
- Effects of TLR9/NF-κB on oxidative stress and inflammation in IPEC-J2 cells.Genes & genomics · 2022Article
- Porcine and Chicken Intestinal Epithelial Cell Models for Screening Phytogenic Feed Additives-Chances and Limitations in Use as Alternatives to Feeding Trials.Microorganisms · 2022Review
- Weaning stress and intestinal health of piglets: A review.Frontiers in immunology · 2022Review
- 4-Phenylbutyric acid accelerates rehabilitation of barrier function in IPEC-J2 cell monolayer model.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
- Protective Effects of Fucoidan against Hydrogen Peroxide-Induced Oxidative Damage in Porcine Intestinal Epithelial Cells.Animals : an open access journal from MDPI · 2019Article
- Rosmarinic Acid, the Main Effective Constituent of Orthosiphon stamineus, Inhibits Intestinal Epithelial Apoptosis Via Regulation of the Nrf2 Pathway in Mice.Molecules (Basel, Switzerland) · 2019Article
- Effects of Quercetin on Proliferation and H₂O₂-Induced Apoptosis of Intestinal Porcine Enterocyte Cells.Molecules (Basel, Switzerland) · 2018Article
- A comparative study of the antioxidant and intestinal protective effects of extracts from different parts of Java tea (Food science & nutrition · 2018Article
- Enrichment of in vivo transcription data from dietary intervention studies with in vitro data provides improved insight into gene regulation mechanisms in the intestinal mucosa.Genes & nutrition · 2017Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously demonstrated that intestinal epithelial cell apoptosis in weaned piglets is much more serious than that observed in sucking piglets and is related to oxidative stress during weaning. It is difficult to study the apoptosis mechanisms only using in vivo methods because of the limit of existing research technology. An in vitro cellular system is required for piglet intestinal epithelial cell apoptosis research. In this study, a non-tumorigenic epithelial cell line, IPEC-J2 cells, was employed as a cell model. Hydrogen peroxide and xanthine/xanthine oxidase (X/XO) were both used and compared for apoptosis modeling. The concentrations of hydrogen peroxide and XO were selected and verified using cell viability analysis, the comet assay and flow cytometry. Intracellular ROS were measured using fluorescent probes. Additionally, the expression levels of the apoptosis-related genes Fas, Bcl-2, P53, Caspase 3, Caspase 8, and Caspase 9 were analyzed using quantitative RT-PCR. The results indicated the optimal modeling method is a final concentration of 0.5 mM H2O2 incubated with IPEC-J2 cells for 1 h at 37 °C in 5 % CO2 for hydrogen peroxide-induced apoptosis modeling, and a final concentration of 250 μM X/50 U/L XO incubated with IPEC-J2 cells for 6 h at 37 °C in 5 % CO2 for X/XO-induced apoptosis modeling. For the apoptotic pathway, the X/XO modeling method is more similar to 21 days weaning piglets. Therefore, we suggest that X/XO modeling with IPEC-J2 cells be used as an in vitro cell culture model for weaning piglet intestinal epithelial cell apoptosis.
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