ArticleApplied and environmental microbiology2015
Integrative conjugative elements are widespread in field isolates of Mycoplasma species pathogenic for ruminants.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 50 citations in OpenAlex.
- Nucleoside binding by a surface lipoprotein governs conjugative ICE acquisition in mycoplasmas.mBio · 2026Article
- Type IV secretion systems: reconciling diversity through a unified nomenclature.FEMS microbiology reviews · 2026Review
- Integrative and conjugative elements inFrontiers in veterinary science · 2026Article
- A versatile plug and play bacterial chassis based on a fast-growing Mycoplasma species.Nucleic acids research · 2025Article
- Interaction with eukaryotic cells enhances the circularization and transcription of integrative and conjugative elements in Mycoplasma hominis.Mobile DNA · 2025Article
- Bacterial conjugation in the ruminant pathogenApplied and environmental microbiology · 2025Article
- Extracellular vesicles of minimalistic Mollicutes as mediators of immune modulation and horizontal gene transfer.Communications biology · 2025Article
- Genomic analysis of the 2017 Aotearoa New Zealand outbreak ofFrontiers in microbiology · 2025Article
- Unraveling the adaptive strategies ofFrontiers in cellular and infection microbiology · 2024Article
- MinisculeSustainable microbiology · 2024Review
- Article
- Impacts ofMicrobial genomics · 2022Article
- Cell-in-Cell Phenomena in Wall-Less Bacteria: Is It Possible?International journal of molecular sciences · 2022Article
- Bovine Respiratory Disease: Conventional to Culture-Independent Approaches to Studying Antimicrobial Resistance in North America.Antibiotics (Basel, Switzerland) · 2022Review
- Genome Mosaicism in Field Strains of Mycoplasma bovis as Footprints of In-Host Horizontal Chromosomal Transfer.Applied and environmental microbiology · 2022Article
- Mycoplasmas as Host Pantropic and Specific Pathogens: Clinical Implications, Gene Transfer, Virulence Factors, and Future Perspectives.Frontiers in cellular and infection microbiology · 2022Review
- Bacterial Conjugation Protocol for Ruminant Mycoplasmas.Bio-protocol · 2021Article
- Serological evidence for historical and present-day exposure of North American bison to Mycoplasma bovis.BMC veterinary research · 2021Article
- Addressing the Antimicrobial Resistance of Ruminant Mycoplasmas Using a Clinical Surveillance Network.Frontiers in veterinary science · 2021Article
- Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comparative genomics have revealed massive horizontal gene transfer (HGT) between Mycoplasma species sharing common ruminant hosts. Further results pointed toward an integrative conjugative element (ICE) as an important contributor of HGT in the small-ruminant-pathogen Mycoplasma agalactiae. To estimate the prevalence of ICEs in ruminant mycoplasmas, we surveyed their occurrence in a collection of 166 field strains representing 4 (sub)species that are recognized as major pathogens. Based on available sequenced genomes, we first defined the conserved, minimal ICE backbone as composed of 4 coding sequences (CDSs) that are evenly distributed and predicted to be essential for ICE chromosomal integration-excision and horizontal transfer. Screening of the strain collection revealed that these 4 CDSs are well represented in ruminant Mycoplasma species, suggesting widespread occurrence of ICEs. Yet their prevalence varies within and among species, with no correlation found with the individual strain history. Extrachromosomal ICE forms were also often detected, suggesting that ICEs are able to circularize in all species, a first and essential step in ICE horizontal transfer. Examination of the junction of the circular forms and comparative sequence analysis of conserved CDSs clearly pointed toward two types of ICE, the hominis and spiroplasma types, most likely differing in their mechanism of excision-integration. Overall, our data indicate the occurrence and maintenance of functional ICEs in a large number of field isolates of ruminant mycoplasmas. These may contribute to genome plasticity and gene exchanges and, presumably, to the emergence of diverse genotypes within pathogenic mycoplasmas of veterinary importance.
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