ArticleApplied and environmental microbiology2015
Mechanisms of methods for hepatitis C virus inactivation.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- Development of a Direct Cell-to-PCR Lysis Buffer Using Optimized Non-Ionic Detergents for RNA-Extraction-Free RT-qPCR.Methods and protocols · 2026Article
- In vitro virucidal activity of chemical compounds against porcine transmissible gastroenteritis virus and porcine respiratory coronavirus.BMC veterinary research · 2025Article
- Modeling of mold inactivation via cold atmospheric plasma (CAP).Applied and environmental microbiology · 2025Article
- Network-based virus dynamic simulation: Evaluating the fomite disinfection effectiveness on SARS-CoV-2 transmission in indoor environment.Infectious Disease Modelling · 2025Article
- Mature dengue virus particles inactivated by a non-ionic detergent retain quaternary neutralizing epitopes and induce potent neutralizing antibodies.Frontiers in immunology · 2025Article
- Mechanisms of action of microbicides commonly used in infection prevention and control.Microbiology and molecular biology reviews : MMBR · 2024Review
- In Vitro Virucidal Activity of Different Essential Oils against Bovine Viral Diarrhea Virus Used as Surrogate of Human Hepatitis C Virus.Antibiotics (Basel, Switzerland) · 2024Article
- Differing Susceptibilities to Certain Microbicidal Chemistries among Three Representative Enveloped Viruses.Microorganisms · 2024Article
- Evaluation of anti-adenoviral effects of the polyvinyl alcohol iodine ophthalmic solution.Japanese journal of ophthalmology · 2024Article
- Kinetics and Monte Carlo simulation of UV disinfection of B. subtilis spores and SARS-CoV-2 in dried saliva droplets.Environmental science and pollution research international · 2021Article
- Inactivation Activities of Ozonated Water, Slightly Acidic Electrolyzed Water and Ethanol against SARS-CoV-2.Molecules (Basel, Switzerland) · 2021Article
- Photo-catalyzed TiOChemical engineering journal (Lausanne, Switzerland : 1996) · 2021Article
- The novel SARS-CoV-2 pandemic: Possible environmental transmission, detection, persistence and fate during wastewater and water treatment.The Science of the total environment · 2021Review
- Review
- A chronicle of SARS-CoV-2: Seasonality, environmental fate, transport, inactivation, and antiviral drug resistance.Journal of hazardous materials · 2021Review
- Point-Of-Care or Point-Of-Need Diagnostic Tests: Time to Change Outbreak Investigation and Pathogen Detection.Tropical medicine and infectious disease · 2020Review
- Control Measures for SARS-CoV-2: A Review on Light-Based Inactivation of Single-Stranded RNA Viruses.Pathogens (Basel, Switzerland) · 2020Review
- Bactericidal and virucidal activity of ethanol and povidone-iodine.MicrobiologyOpen · 2020Review
- Decontamination of N95 masks for re-use employing 7 widely available sterilization methods.PloS one · 2020Article
- IL-1β/TNF-α/IL-6 inflammatory cytokines promote STAT1-dependent induction of CH25H in Zika virus-infected human macrophages.The Journal of biological chemistry · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Virus inactivation by chemical disinfectants is an important instrument for infection control in medical settings, but the mechanisms involved are poorly understood. In this study, we systematically investigated the effects of several antiviral treatments on hepatitis C virus (HCV) particles as model for enveloped viruses. Studies were performed with authentic cell culture-derived viruses, and the influence of chemical disinfectants, heat, and UV treatment on HCV was analyzed by the determination of infectious particles in a limiting-dilution assay, by quantitative reverse transcription-PCR, by core enzyme-linked immunosorbent assay, and by proteolytic protection assay. All different inactivation methods resulted in a loss of HCV infectivity by targeting different parts of the virus particle. Alcohols such as ethanol and 2-propanol did not affect the viral RNA genome integrity but disrupted the viral envelope membrane in a capsid protection assay. Heat and UV treatment of HCV particles resulted in direct damage of the viral genome since transfection of viral particle-associated RNA into permissive cells did not initiate RNA replication. In addition, heat incubation at 80°C disrupted the HCV envelope, rendering the viral capsid susceptible to proteolytic digest. This study demonstrated the molecular processes of viral inactivation of an enveloped virus and should facilitate the development of effective disinfection strategies in infection control not only against HCV but also against other enveloped viruses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.